Overview
- External ontology
- TOGO:M614 — Halomonas Subglaciescola Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M614 Original source: JCM - JCM_M607 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=607
Composition
flowchart LR
CultureMech_010014["halomonas_subglaciescola_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010014 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010014 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_010014 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_010014 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010014 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010014 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_010014 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_010014 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_KNO3["KNO3"]
CultureMech_010014 --> ing8_KNO3
CHEBI_63043["CHEBI:63043"]
ing8_KNO3 -.-> CHEBI_63043
ing9_Peptone["Peptone"]
CultureMech_010014 --> ing9_Peptone
ing10_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010014 --> ing10_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_2H2O -.-> CHEBI_75213
ing11_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010014 --> ing11_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_7H2O -.-> CHEBI_75836
ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_010014 --> ing12_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
ing13_KOH["KOH"]
CultureMech_010014 --> ing13_KOH
CHEBI_32035["CHEBI:32035"]
ing13_KOH -.-> CHEBI_32035
ing14_KH2PO4["KH2PO4"]
CultureMech_010014 --> ing14_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing14_KH2PO4 -.-> CHEBI_63036
ing15_K2HPO4["K2HPO4"]
CultureMech_010014 --> ing15_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing15_K2HPO4 -.-> CHEBI_131527
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
39.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 9.5, 29.7] |
| 3 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.2, 3.3] |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 | Peptone | 5 G_PER_L | ||
| 11 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
12.7 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
99 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 14 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 15 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Metal solution (see below)
Dose: 20 G_PER_L
Phosphate solution (see below)
Dose: 20 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Metals ''44'' (see Medium [M140])
Dose: 50 G_PER_L
Cross-reference to Medium M140
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.407672Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M614 |
| 2026-01-28T19:48:09.421203Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:15.251142+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:42.433874Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:10.725696Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010014 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.652352+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halomonas Subglaciescola Medium" to "halomonas_subglaciescola_medium" for consistent matching |
| 2026-05-16T09:03:19.544597+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.015817+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.849488+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.159993+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:51.084871+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |