Overview
- External ontology
- TOGO:M616 — Halomonas Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M616 Original source: JCM - JCM_M608 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=608
Composition
flowchart LR
CultureMech_010016["halomonas_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010016 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010016 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_010016 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_010016 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2・6H2O"]
CultureMech_010016 --> ing4_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing4_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing5_Sodium_citrate["Sodium citrate"]
CultureMech_010016 --> ing5_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Sodium_citrate -.-> CHEBI_53258
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010016 --> ing6_Yeast_extract__BD_Difco_
ing7_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_010016 --> ing7_Proteose_peptone_No__3__BD_Difco_
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010016 --> ing8_Casamino_acids__BD_Difco_
ing9_KOH["KOH"]
CultureMech_010016 --> ing9_KOH
CHEBI_32035["CHEBI:32035"]
ing9_KOH -.-> CHEBI_32035
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
80 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
Fe(NH4)2(SO4)2・6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.05 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 7 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 8 | Proteose peptone No. 3 (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 9 | Casamino acids (BD-Difco) | 7.5 G_PER_L | Properties: Undefined component | |
| 10 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.412101Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M616 |
| 2026-01-28T19:48:14.987387Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-13T06:20:10.742550Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010016 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.667973+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halomonas Medium" to "halomonas_medium" for consistent matching |
| 2026-05-16T09:03:19.562836+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:43.034030+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.869319+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.169192+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |