sphaerotilus_medium

CultureMech:010017 bacterial COMPLEX SOLID_AGAR
Original name: Sphaerotilus Medium

Overview

External ontology
TOGO:M618 — Sphaerotilus Medium
Applications
  • Microbial cultivation
pH
7.0
Notes
JCM Medium 610 Sphaerotilus Medium lists 0.5 g Tryptone (BD-Difco), 1.0 g Glycerol, 0.1 g Yeast extract (BD-Difco), 10.0 g Agar if needed, and 1.0 L Distilled water, adjusted to pH 7.0.

Composition

flowchart LR
    CultureMech_010017["sphaerotilus_medium"]
    ing0_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_010017 --> ing0_Tryptone__BD_Difco_
    ing1_Glycerol["Glycerol"]
    CultureMech_010017 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010017 --> ing2_Yeast_extract__BD_Difco_
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_010017 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_010017 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Tryptone (BD-Difco) 0.5 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
 TOGO M618 / JCM Medium 610 lists 0.5 g/L Tryptone (BD-Difco).
2 Glycerol
C3H8O3 92.094 g/mol
1.0 G_PER_L CHEBI:17754
glycerol
 TOGO M618 / JCM Medium 610 lists 1.0 g/L Glycerol.
3 Yeast extract (BD-Difco) 0.1 G_PER_L FOODON:03315426
yeast extract
 TOGO M618 / JCM Medium 610 lists 0.1 g/L Yeast extract (BD-Difco).
4 Agar (if needed) 10.0 G_PER_L CHEBI:2509
agar
 TOGO M618 / JCM Medium 610 lists 10 g/L Agar if needed.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M618 / JCM Medium 610 lists 1.0 L Distilled water.

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.413548Z togo-import Imported from TOGO Medium Source: JCM, ID: M618
2026-01-28T19:48:41.130904Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:10.747411Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010017
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.672558+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Sphaerotilus Medium" to "sphaerotilus_medium" for consistent matching
2026-06-05T07:40:43.039597+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:34.751661+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-09-11T00:00:00-07:00 repair_togo_m618_score15.py RESOLVED_TOGO_M618_SCORE15 Corrected the imported distilled-water volume, added pH 7.0 from JCM Medium 610, grounded tryptone, yeast extract, glycerol, agar, and water, added JCM autoclave metadata, and linked MediaDive J610 as a source duplicate.