cys_medium_for_ymo722

CultureMech:010028 bacterial COMPLEX LIQUID
Original name: CYS Medium For YMO722

Overview

External ontology
TOGO:M628 — CYS Medium For YMO722
Applications
  • Microbial cultivation
pH
7.5
Notes
JCM Medium 619 CYS Medium for YMO722 uses JCM Medium 618 with 16.0 g/L final NaCl.

Composition

flowchart LR
    CultureMech_010028["cys_medium_for_ymo722"]
    ing0_N_Z_Case__Wako_["N-Z-Case (Wako)"]
    CultureMech_010028 --> ing0_N_Z_Case__Wako_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010028 --> ing1_Yeast_extract__BD_Difco_
    ing2_Soluble_starch__BD_Difco_["Soluble starch (BD-Difco)"]
    CultureMech_010028 --> ing2_Soluble_starch__BD_Difco_
    CHEBI_28017["CHEBI:28017"]
    ing2_Soluble_starch__BD_Difco_ -.-> CHEBI_28017
    ing3_NaCl["NaCl"]
    CultureMech_010028 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_010028 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_010028 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_010028 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_Distilled_water["Distilled water"]
    CultureMech_010028 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 N-Z-Case (Wako) 3.0 G_PER_L TOGO M628 / JCM Medium 619 lists 3.0 g/L N-Z-Case (Wako).
2 Yeast extract (BD-Difco) 2.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M628 / JCM Medium 619 lists 2.0 g/L Yeast extract (BD-Difco).
3 Soluble starch (BD-Difco) 1.0 G_PER_L CHEBI:28017
starch
 TOGO M628 / JCM Medium 619 lists 1.0 g/L Soluble starch (BD-Difco).
4 NaCl
Cl.Na 58.443 g/mol
16.0 G_PER_L CHEBI:26710
sodium chloride
 TOGO M628 / JCM Medium 619 lists 16.0 g/L NaCl.
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.125 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 TOGO M628 / JCM Medium 619 lists 0.125 g/L MgCl2 x 6 H2O.
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.025 G_PER_L CHEBI:86158
calcium chloride dihydrate
 TOGO M628 / JCM Medium 619 lists 0.025 g/L CaCl2 x 2 H2O.
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 TOGO M628 / JCM Medium 619 lists 0.01 g/L FeSO4 x 7 H2O.
8 Distilled water
H2O 18.015 g/mol
900.0 ML_PER_L CHEBI:15377
water
 TOGO M628 / JCM Medium 619 lists 900 ml distilled water before pH adjustment.

Preparation

  1. Add the base components to 900 ml distilled water and mix thoroughly.
  2. Add each trace-metal stock solution at 0.1 ml/L in the listed order.
  3. Adjust pH to 7.5, bring the volume to 1.0 L with distilled water, and autoclave.
  4. Inoculate into the medium prewarmed to the optimal growth temperature.

Solutions

Na2MoO4 x 2 H2O stock

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2MoO4 x 2 H2O 12.0 G_PER_L Na2MoO4 x 2 H2O stock is represented as a 12.0 g/L Na2MoO4 x 2 H2O stock.
TOGO M628 / JCM Medium 619 adds 100 uL of 12.0 g/L Na2MoO4 x 2 H2O.

VOSO4 x n H2O stock

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
VOSO4 x n H2O 1.0 G_PER_L VOSO4 x n H2O stock is represented as a 1.0 g/L VOSO4 x n H2O stock.
TOGO M628 / JCM Medium 619 adds 100 uL of 1.0 g/L VOSO4 x n H2O.

MnCl2 x 4 H2O stock

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
MnCl2 x 4 H2O 5.0 G_PER_L MnCl2 x 4 H2O stock is represented as a 5.0 g/L MnCl2 x 4 H2O stock in 0.01 N H2SO4.
TOGO M628 / JCM Medium 619 adds 100 uL of 5.0 g/L MnCl2 x 4 H2O in 0.01 N H2SO4.

ZnSO4 x 7 H2O stock

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 0.6 G_PER_L ZnSO4 x 7 H2O stock is represented as a 0.6 g/L ZnSO4 x 7 H2O stock in 0.01 N H2SO4.
TOGO M628 / JCM Medium 619 adds 100 uL of 0.6 g/L ZnSO4 x 7 H2O in 0.01 N H2SO4.

CuSO4 x 5 H2O stock

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
CuSO4 x 5 H2O 0.15 G_PER_L CuSO4 x 5 H2O stock is represented as a 0.15 g/L CuSO4 x 5 H2O stock in 0.01 N H2SO4.
TOGO M628 / JCM Medium 619 adds 100 uL of 0.15 g/L CuSO4 x 5 H2O in 0.01 N H2SO4.

CoCl2 x 6 H2O stock

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
CoCl2 x 6 H2O 8.0 G_PER_L CoCl2 x 6 H2O stock is represented as a 8.0 g/L CoCl2 x 6 H2O stock in 0.01 N H2SO4.
TOGO M628 / JCM Medium 619 adds 100 uL of 8.0 g/L CoCl2 x 6 H2O in 0.01 N H2SO4.

NiCl2 x 6 H2O stock

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
NiCl2 x 6 H2O 0.2 G_PER_L NiCl2 x 6 H2O stock is represented as a 0.2 g/L NiCl2 x 6 H2O stock in 0.01 N H2SO4.
TOGO M628 / JCM Medium 619 adds 100 uL of 0.2 g/L NiCl2 x 6 H2O in 0.01 N H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.428386Z togo-import Imported from TOGO Medium Source: JCM, ID: M628
2026-01-28T19:48:06.957530Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:10.811403Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010028
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.737428+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CYS Medium For YMO722" to "cys_medium_for_ymo722" for consistent matching
2026-06-05T07:40:43.097133+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m627_m628_score15.py RESOLVED_TOGO_M628_SCORE15 Corrected the imported distilled-water and trace-stock units in the JCM Medium 619 16.0 g/L NaCl variant, represented the seven 100 uL trace-metal additions as stock solutions, grounded soluble starch, yeast extract, and the trace salts, retained N-Z-Case as intentionally unmapped, and linked MediaDive J619 as a source duplicate.