Overview
- External ontology
- TOGO:M631 — HYL Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M631 Original source: JCM - JCM_M621 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=621
Composition
flowchart LR
CultureMech_010032["hyl_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010032 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010032 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_010032 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010032 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_010032 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_010032 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_010032 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010032 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_010032 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Na2SO4["Na2SO4"]
CultureMech_010032 --> ing9_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing9_Na2SO4 -.-> CHEBI_32149
ing10_HEPES["HEPES"]
CultureMech_010032 --> ing10_HEPES
CHEBI_46756["CHEBI:46756"]
ing10_HEPES -.-> CHEBI_46756
ing11_N2["N2"]
CultureMech_010032 --> ing11_N2
CHEBI_17997["CHEBI:17997"]
ing11_N2 -.-> CHEBI_17997
ing12_0_2__Fe_NH4_2_SO4__6H2O__in_0_037__HCl_["0.2% Fe(NH4)2(SO4)・6H2O (in 0.037% HCl)"]
CultureMech_010032 --> ing12_0_2__Fe_NH4_2_SO4__6H2O__in_0_037__HCl_
ing13_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010032 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_010032 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010032 --> ing15_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2_6H2O -.-> CHEBI_35696
ing16_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010032 --> ing16_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_7H2O -.-> CHEBI_32312
ing17_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010032 --> ing17_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_5H2O -.-> CHEBI_31440
ing18_KAl_SO4_2_12H2O["KAl(SO4)2・12H2O"]
CultureMech_010032 --> ing18_KAl_SO4_2_12H2O
CHEBI_86465["CHEBI:86465"]
ing18_KAl_SO4_2_12H2O -.-> CHEBI_86465
ing19_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_010032 --> ing19_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_2H2O -.-> CHEBI_63939
ing20_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010032 --> ing20_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing20_MnSO4_xH2O -.-> CHEBI_86360
ing21_Na2SeO3["Na2SeO3"]
CultureMech_010032 --> ing21_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing21_Na2SeO3 -.-> CHEBI_48843
ing22_NiSO4_6H2O["NiSO4・6H2O"]
CultureMech_010032 --> ing22_NiSO4_6H2O
CHEBI_53001["CHEBI:53001"]
ing22_NiSO4_6H2O -.-> CHEBI_53001
ing23_EDTA_2Na["EDTA・2Na"]
CultureMech_010032 --> ing23_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing23_EDTA_2Na -.-> CHEBI_64734
ing24_KOH["KOH"]
CultureMech_010032 --> ing24_KOH
CHEBI_32035["CHEBI:32035"]
ing24_KOH -.-> CHEBI_32035
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.34 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | HEPES | 4.76 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 12 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 13 | 0.2% Fe(NH4)2(SO4)・6H2O (in 0.037% HCl) | 1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Solution | |
| 14 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
1.8 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
KAl(SO4)2・12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.1 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
3.26 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.05 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
NiSO4・6H2O
Ni.O4S 154.756 g/mol
|
0.11 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
29 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 25 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
Sodium lactate solution (50%)
Dose: 4.5 G_PER_L
Role: Carbon source; Properties: Defined component, Organic compound, Solution
0.5 M Na2S・9H2O solution
Dose: 4 G_PER_L
Role: Reducing agent; Properties: Inorganic compound, Solution, Defined component
Trace elements (see below)
Dose: 1 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.434288Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M631 |
| 2026-01-28T19:48:13.009056Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:21.507684+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:49.588933Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:10.837511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010032 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.764225+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYL Medium" to "hyl_medium" for consistent matching |
| 2026-05-16T09:03:19.633934+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.126047+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.194381+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:51.205093+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |