mmb_medium

CultureMech:010046 bacterial COMPLEX SOLID_AGAR
Original name: MMB Medium

Overview

External ontology
TOGO:M645 — MMB Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M645 Original source: JCM - JCM_M631-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=631

Composition

flowchart LR
    CultureMech_010046["mmb_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010046 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010046 --> ing1_Yeast_extract
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010046 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_Glucose["Glucose"]
    CultureMech_010046 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Peptone["Peptone"]
    CultureMech_010046 --> ing4_Peptone
    ing5_agar["agar"]
    CultureMech_010046 --> ing5_agar
    CHEBI_2509["CHEBI:2509"]
    ing5_agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Yeast extract 0.1 G_PER_L Role: Nutrient source; Properties: Undefined component
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.2 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
4 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
5 Peptone 0.1 G_PER_L
6 agar variable VARIABLE CHEBI:2509
agar
 Role: Solidifying component

Solutions

Hunter's basal salts (see Medium [M644])

Dose: 20 G_PER_L

Cross-reference to Medium M644

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.456989Z togo-import Imported from TOGO Medium Source: JCM, ID: M645
2026-01-28T19:48:09.821910Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: agar
2026-03-09T08:04:15.951633+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:10.929260Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010046
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.857385+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMB Medium" to "mmb_medium" for consistent matching
2026-05-16T09:03:19.734601+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:43.225123+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.228567+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.