cellulosilyticum_ruminicola_medium

CultureMech:010055 bacterial COMPLEX LIQUID
Original name: Cellulosilyticum Ruminicola Medium

Overview

External ontology
TOGO:M653 — Cellulosilyticum Ruminicola Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M653 Original source: JCM - JCM_M639 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=639

Composition

flowchart LR
    CultureMech_010055["cellulosilyticum_ruminicola_medium"]
    ing0_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
    CultureMech_010055 --> ing0_L__cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing0_L__cysteine_HCl_H2O -.-> CHEBI_91248
    ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010055 --> ing1_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing1_Carbon_dioxide_gas -.-> CHEBI_16526
    ing2_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010055 --> ing2_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing2_Nitrogen_gas -.-> CHEBI_17997
    ing3_Distilled_water["Distilled water"]
    CultureMech_010055 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010055 --> ing4_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_7H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_010055 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010055 --> ing6_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_2H2O -.-> CHEBI_86158
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010055 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_Cellobiose["Cellobiose"]
    CultureMech_010055 --> ing8_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing8_Cellobiose -.-> CHEBI_17057
    ing9_KH2PO4["KH2PO4"]
    CultureMech_010055 --> ing9_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing9_KH2PO4 -.-> CHEBI_63036
    ing10_K2HPO4["K2HPO4"]
    CultureMech_010055 --> ing10_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing10_K2HPO4 -.-> CHEBI_131527
    ing11_NaHCO3["NaHCO3"]
    CultureMech_010055 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
variable VARIABLE CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Solution
2 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
3 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
4 Distilled water
H2O 18.015 g/mol
800.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 600.0, 100.0, 100.0]
5 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.9 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 NaCl
Cl.Na 58.443 g/mol
0.9 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.9 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.9 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
9 Cellobiose
C12H22O11 342.297 g/mol
2.5 G_PER_L CHEBI:17057
cellobiose
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
10 KH2PO4
H2O4P.K 136.084 g/mol
0.45 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
11 K2HPO4
HO4P.2K 174.174 g/mol
0.45 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
12 NaHCO3
CHO3.Na 84.006 g/mol
3.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component

Solutions

Solution A

Dose: 800 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 100 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Solution C

Dose: 100 G_PER_L

See mediadive_5312_Solution_C.yaml for composition

Rumen fluid, clarified (see Medium [M258])

Dose: 200 G_PER_L

Cross-reference to Medium M258

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.471879Z togo-import Imported from TOGO Medium Source: JCM, ID: M653
2026-01-28T19:48:19.366955Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: L--cysteine・HCl・H2O; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:32.066659+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:09:01.266793Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:11.000179Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010055
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.928329+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Cellulosilyticum Ruminicola Medium" to "cellulosilyticum_ruminicola_medium" for consistent matching
2026-05-16T09:03:19.799159+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:43.294707+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:32.102053+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.253879+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.