Overview
- External ontology
- TOGO:M656 — Moorella AN10 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M656 Original source: JCM - JCM_M642 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=642
Composition
flowchart LR
CultureMech_010058["moorella_an10_medium"]
ing0_Na2S_9H2O["Na2S・9H2O"]
CultureMech_010058 --> ing0_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing0_Na2S_9H2O -.-> CHEBI_76209
ing1_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
CultureMech_010058 --> ing1_L__cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing1_L__cysteine_HCl_H2O -.-> CHEBI_91248
ing2_N2["N2"]
CultureMech_010058 --> ing2_N2
CHEBI_17997["CHEBI:17997"]
ing2_N2 -.-> CHEBI_17997
ing3_Distilled_water["Distilled water"]
CultureMech_010058 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010058 --> ing4_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_7H2O -.-> CHEBI_31795
ing5_Yeast_extract["Yeast extract"]
CultureMech_010058 --> ing5_Yeast_extract
ing6_NaCl["NaCl"]
CultureMech_010058 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010058 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_KH2PO4["KH2PO4"]
CultureMech_010058 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_NH4Cl["NH4Cl"]
CultureMech_010058 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_K2HPO4["K2HPO4"]
CultureMech_010058 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_Resazurin["Resazurin"]
CultureMech_010058 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_FeSO4_6H2O["FeSO4・6H2O"]
CultureMech_010058 --> ing12_FeSO4_6H2O
CHEBI_75832["CHEBI:75832"]
ing12_FeSO4_6H2O -.-> CHEBI_75832
ing13_Casitone__BD_Difco_["Casitone (BD-Difco)"]
CultureMech_010058 --> ing13_Casitone__BD_Difco_
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010058 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_Nitrogen_gas["Nitrogen gas"]
CultureMech_010058 --> ing15_Nitrogen_gas
ing15_Nitrogen_gas -.-> CHEBI_17997
ing16_Fructose["Fructose"]
CultureMech_010058 --> ing16_Fructose
CHEBI_28645["CHEBI:28645"]
ing16_Fructose -.-> CHEBI_28645
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.8 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.3, 0.5] |
| 2 |
L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.8 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound [Merged 2 duplicates: 0.3, 0.5] |
| 3 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 4 |
Distilled water
H2O 18.015 g/mol
|
950.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 850.0, 100.0] |
| 5 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
2.25 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.227 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.348 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 13 |
FeSO4・6H2O
Fe.O4S 151.908 g/mol
|
0.1 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | Casitone (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 16 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 |
Fructose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 862 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 20 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Trace element solution (see Medium [M481])
Dose: 1 G_PER_L
Cross-reference to Medium M481
Se/W solution (see Medium [M312])
Dose: 1 G_PER_L
Cross-reference to Medium M312
8% NaHCO3 solution
Dose: 20 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.478840Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M656 |
| 2026-01-28T19:48:11.409934Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:18.672657+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:08:46.404099Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:20:11.034115Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010058 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.961058+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Moorella AN10 Medium" to "moorella_an10_medium" for consistent matching |
| 2026-05-16T09:03:19.837735+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.332220+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.136636+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.269115+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |