Overview
- External ontology
- TOGO:M659 — Halosarcina Pallida Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M659 Original source: JCM - JCM_M645 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=645
Composition
flowchart LR
CultureMech_010061["halosarcina_pallida_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010061 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010061 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_010061 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010061 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010061 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_010061 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010061 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_K2SO4["K2SO4"]
CultureMech_010061 --> ing7_K2SO4
CHEBI_32036["CHEBI:32036"]
ing7_K2SO4 -.-> CHEBI_32036
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010061 --> ing8_Casamino_acids__BD_Difco_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 9 | Casamino acids (BD-Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.486573Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M659 |
| 2026-01-28T19:48:43.087181Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:11.059462Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010061 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.985786+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halosarcina Pallida Medium" to "halosarcina_pallida_medium" for consistent matching |
| 2026-06-05T07:40:43.356932+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.281590+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |