Overview
- External ontology
- TOGO:M65 — Nutrient Agar
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- JCM Medium 74 / Nutrient Agar lists 1.0 L distilled water, 5.0 g/L peptone, 3.0 g/L beef extract, and 15.0 g/L agar, adjusted to pH 7.0; TOGO M65 and MediaDive J74 report the same JCM recipe.
Composition
flowchart LR
CultureMech_010062["nutrient_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_010062 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Peptone["Peptone"]
CultureMech_010062 --> ing1_Peptone
ing2_Beef_extract["Beef extract"]
CultureMech_010062 --> ing2_Beef_extract
ing3_Agar["Agar"]
CultureMech_010062 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M65 / JCM Medium 74 lists 1.0 L Distilled water. |
| 2 | Peptone | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
TOGO M65 / JCM Medium 74 lists 5.0 g/L Peptone. |
| 3 | Beef extract | 3.0 G_PER_L |
FOODON:03302088
Beef extract
|
TOGO M65 / JCM Medium 74 lists 3.0 g/L Beef extract. |
| 4 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M65 / JCM Medium 74 lists 15.0 g/L Agar. |
Preparation
- Dissolve 5.0 g/L peptone, 3.0 g/L beef extract, and 15.0 g/L agar in 1.0 L distilled water.
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.464612Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M65 |
| 2026-01-28T19:48:32.450067Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:11.063608Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010062 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.990065+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient Agar" to "nutrient_agar" for consistent matching |
| 2026-06-05T07:40:43.362018+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m92_alkaline_nutrient_score15.py | RESOLVED_TOGO_M65_NUTRIENT_AGAR | Corrected water from 1 g/L to 1.0 L, grounded peptone and beef extract, added the pH 7.0 adjustment from TOGO M65 and MediaDive J74, and linked the MediaDive J74 source duplicate. |