Overview
- External ontology
- TOGO:M666 — SAP2 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M666 Original source: JCM - JCM_M651 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=651
Composition
flowchart LR
CultureMech_010069["sap2_medium"]
ing0_Agar["Agar"]
CultureMech_010069 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010069 --> ing1_Yeast_extract__BD_Difco_
ing2_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010069 --> ing2_Tryptone__BD_Difco_
ing3_0_5_x_Artificial_seawater__see_below_["0.5 x Artificial seawater (see below)"]
CultureMech_010069 --> ing3_0_5_x_Artificial_seawater__see_below_
ing4_Distilled_water["Distilled water"]
CultureMech_010069 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010069 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_010069 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010069 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010069 --> ing8_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_6H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_010069 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 2 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 3 | Tryptone (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 4 | 0.5 x Artificial seawater (see below) | 1 G_PER_L | ||
| 5 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.35 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.498635Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M666 |
| 2026-01-28T19:48:15.088243Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:11.107867Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010069 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.034132+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SAP2 Medium" to "sap2_medium" for consistent matching |
| 2026-06-05T07:40:43.411495+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.203578+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |