pelagicoccus_agar

CultureMech:010079 bacterial COMPLEX SOLID_AGAR
Original name: Pelagicoccus Agar

Overview

External ontology
TOGO:M675 — Pelagicoccus Agar
Applications
  • Microbial cultivation
pH
7.5
Notes
JCM Medium 659 Pelagicoccus Agar lists 55.1 g Marine agar 2216 (BD-Difco), 9.1 g R2A agar (BD-Difco), 750.0 ml Artificial seawater, and 250.0 ml Distilled water, then adjusts pH to 7.5.

Composition

flowchart LR
    CultureMech_010079["pelagicoccus_agar"]
    ing0_Marine_agar_2216__BD_Difco_["Marine agar 2216 (BD-Difco)"]
    CultureMech_010079 --> ing0_Marine_agar_2216__BD_Difco_
    ing1_R2A_agar__BD_Difco_["R2A agar (BD-Difco)"]
    CultureMech_010079 --> ing1_R2A_agar__BD_Difco_
    ing2_Distilled_water["Distilled water"]
    CultureMech_010079 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Marine agar 2216 (BD-Difco) 55.1 G_PER_L TOGO M675 / JCM Medium 659 lists 55.1 g/L Marine agar 2216 (BD-Difco); this commercial medium product is retained as an opaque complex component.
2 R2A agar (BD-Difco) 9.1 G_PER_L TOGO M675 / JCM Medium 659 lists 9.1 g/L R2A agar (BD-Difco); this commercial medium product is retained as an opaque complex component.
3 Distilled water
H2O 18.015 g/mol
250.0 ML_PER_L CHEBI:15377
water
 TOGO M675 / JCM Medium 659 lists 250.0 ml/L Distilled water.

Preparation

  1. Combine the listed commercial medium components with 750.0 ml Artificial seawater and 250.0 ml distilled water.
  2. Adjust pH to 7.5.

Solutions

Artificial seawater

Dose: 750.0 ML_PER_L

CultureMech:013514

Stock composition
IngredientConcentrationNotes
NaCl 24.0 G_PER_L JCM Medium 659 lists 24.0 g/L NaCl.
MgSO4 x 7 H2O 7.0 G_PER_L JCM Medium 659 lists 7.0 g/L MgSO4 x 7 H2O.
MgCl2 x 6 H2O 5.3 G_PER_L JCM Medium 659 lists 5.3 g/L MgCl2 x 6 H2O.
KCl 0.7 G_PER_L JCM Medium 659 lists 0.7 g/L KCl.
CaCl2 x 2 H2O 0.1 G_PER_L JCM Medium 659 lists 0.1 g/L CaCl2 x 2 H2O.
Distilled water 1.0 L JCM Medium 659 lists 1.0 L Distilled water in the Artificial seawater stock solution.
JCM Medium 659 adds 750.0 ml/L Artificial seawater prepared from the printed subrecipe.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.510041Z togo-import Imported from TOGO Medium Source: JCM, ID: M675
2026-01-28T19:48:08.059163Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:08:39.902130Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:11.159597Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010079
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.084933+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Pelagicoccus Agar" to "pelagicoccus_agar" for consistent matching
2026-06-05T07:40:43.470144+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:32.259429+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-09-11T00:00:00-07:00 repair_togo_m675_m676_score15.py RESOLVED_TOGO_M675_SCORE15 Corrected the imported distilled-water and Artificial seawater units, moved Artificial seawater from flattened ingredients to a 750.0 ml/L inline stock solution, added pH 7.5, de-grounded the commercial Marine agar and R2A agar products, and linked the JCM 659 source duplicate.