pelagicoccus_agar

CultureMech:010080 bacterial COMPLEX LIQUID
Original name: Pelagicoccus Agar

Overview

External ontology
TOGO:M676 — Pelagicoccus Agar
Applications
  • Microbial cultivation
pH
7.5
Notes
JCM Medium 659 directs the liquid medium to dissolve 37.4 g/L Marine Broth 2216 (BD-Difco) and 1.6 g/L R2A broth (Daigo) in the artificial sea water; TOGO M676 imports that liquid form with 750.0 ml/L Artificial seawater and 250.0 ml/L Distilled water.

Composition

flowchart LR
    CultureMech_010080["pelagicoccus_agar"]
    ing0_Marine_broth_2216__BD_Difco_["Marine broth 2216 (BD-Difco)"]
    CultureMech_010080 --> ing0_Marine_broth_2216__BD_Difco_
    ing1_R2A_broth__Daigo_["R2A broth (Daigo)"]
    CultureMech_010080 --> ing1_R2A_broth__Daigo_
    ing2_Distilled_water["Distilled water"]
    CultureMech_010080 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Marine broth 2216 (BD-Difco) 37.4 G_PER_L TOGO M676 / JCM Medium 659 liquid form lists 37.4 g/L Marine broth 2216 (BD-Difco); this commercial medium product is retained as an opaque complex component.
2 R2A broth (Daigo) 1.6 G_PER_L TOGO M676 / JCM Medium 659 liquid form lists 1.6 g/L R2A broth (Daigo); this commercial medium product is retained as an opaque complex component.
3 Distilled water
H2O 18.015 g/mol
250.0 ML_PER_L CHEBI:15377
water
 TOGO M676 / JCM Medium 659 liquid form lists 250.0 ml/L Distilled water.

Preparation

  1. Combine the listed commercial medium components with 750.0 ml Artificial seawater and 250.0 ml distilled water.
  2. Adjust pH to 7.5.

Solutions

Artificial seawater

Dose: 750.0 ML_PER_L

CultureMech:013514

Stock composition
IngredientConcentrationNotes
NaCl 24.0 G_PER_L JCM Medium 659 lists 24.0 g/L NaCl.
MgSO4 x 7 H2O 7.0 G_PER_L JCM Medium 659 lists 7.0 g/L MgSO4 x 7 H2O.
MgCl2 x 6 H2O 5.3 G_PER_L JCM Medium 659 lists 5.3 g/L MgCl2 x 6 H2O.
KCl 0.7 G_PER_L JCM Medium 659 lists 0.7 g/L KCl.
CaCl2 x 2 H2O 0.1 G_PER_L JCM Medium 659 lists 0.1 g/L CaCl2 x 2 H2O.
Distilled water 1.0 L JCM Medium 659 lists 1.0 L Distilled water in the Artificial seawater stock solution.
JCM Medium 659 adds 750.0 ml/L Artificial seawater prepared from the printed subrecipe.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.511516Z togo-import Imported from TOGO Medium Source: JCM, ID: M676
2026-01-28T19:48:15.099175Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:25.116422+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:11.164425Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010080
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.089725+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Pelagicoccus Agar" to "pelagicoccus_agar" for consistent matching
2026-05-16T09:03:19.969655+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:43.475596+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m675_m676_score15.py RESOLVED_TOGO_M676_SCORE15 Corrected the imported distilled-water and Artificial seawater units, replaced the empty Artificial seawater cross-reference with a 750.0 ml/L inline stock solution, added pH 7.5, kept the commercial Marine broth and R2A broth products ungrounded, and linked the JCM 659 agar parent as a physical-state variant.