mbg_20

CultureMech:010085 bacterial COMPLEX LIQUID
Original name: MBG-20

Overview

External ontology
TOGO:M680 — MBG-20
Applications
  • Microbial cultivation
pH
7.0
Notes
JCM Medium 662 MBG-20 lists 16.0 g Peptone, 4.0 g Yeast extract, 5.0 g Glucose, 40.0 g Sea salts (Sigma), and 1.0 L Distilled water, then adjusts pH to 7.0.

Composition

flowchart LR
    CultureMech_010085["mbg_20"]
    ing0_Peptone["Peptone"]
    CultureMech_010085 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010085 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_010085 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_010085 --> ing3_Sea_salts__Sigma_
    ing4_Distilled_water["Distilled water"]
    CultureMech_010085 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 16.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 TOGO M680 / JCM Medium 662 lists 16.0 g/L Peptone.
2 Yeast extract 4.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M680 / JCM Medium 662 lists 4.0 g/L Yeast extract.
3 Glucose
C6H12O6 180.156 g/mol
5.0 G_PER_L CHEBI:17234
glucose
 TOGO M680 / JCM Medium 662 lists 5.0 g/L Glucose.
4 Sea salts (Sigma) 40.0 G_PER_L TOGO M680 / JCM Medium 662 lists 40.0 g/L Sea salts (Sigma); this commercial sea-salt mixture is retained as an opaque complex component.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M680 / JCM Medium 662 lists 1.0 L Distilled water.

Preparation

  1. Adjust pH to 7.0.
  2. Dissolve Sea salts (Sigma) in distilled water.
  3. Sterilize the Sea salts solution by filtration.
  4. Separately autoclave Peptone, Yeast extract, and Glucose solutions.
  5. Add the sterile Peptone, Yeast extract, and Glucose solutions to the sterile Sea salts solution.
  6. Make the liquid medium anaerobic by repeated cycles, three times, of degassing and charging with argon.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.518952Z togo-import Imported from TOGO Medium Source: JCM, ID: M680
2026-01-28T19:48:38.819373Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:11.193900Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010085
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.119504+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MBG-20" to "mbg_20" for consistent matching
2026-06-05T07:40:43.510149+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m680_score15.py RESOLVED_TOGO_M680_SCORE15 Corrected the imported distilled-water unit, added pH 7.0, grounded Peptone, Yeast extract, and Glucose, kept Sea salts (Sigma) intentionally ungrounded, added the split filtration, autoclaving, and argon preparation instructions, and linked the JCM 662 source duplicate.