Overview
- External ontology
- TOGO:M681 — Nutrient Broth
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- JCM Medium 663 Nutrient Broth lists 5.0 g Peptone, 3.0 g Beef extract, and 1.0 L Distilled water, then adjusts pH to 7.0.
Composition
flowchart LR
CultureMech_010086["nutrient_broth"]
ing0_Peptone["Peptone"]
CultureMech_010086 --> ing0_Peptone
ing1_Beef_extract["Beef extract"]
CultureMech_010086 --> ing1_Beef_extract
ing2_Distilled_water["Distilled water"]
CultureMech_010086 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
TOGO M681 / JCM Medium 663 lists 5.0 g/L Peptone. |
| 2 | Beef extract | 3.0 G_PER_L |
FOODON:03302088
beef extract
|
TOGO M681 / JCM Medium 663 lists 3.0 g/L Beef extract. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M681 / JCM Medium 663 lists 1.0 L Distilled water. |
Preparation
- Dissolve 5.0 g Peptone and 3.0 g Beef extract in 1.0 L distilled water.
- Adjust pH to 7.0.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.519876Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M681 |
| 2026-01-28T19:48:29.123817Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:11.197453Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010086 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.122917+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient Broth" to "nutrient_broth" for consistent matching |
| 2026-06-05T07:40:43.513701+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m681_score15.py | RESOLVED_TOGO_M681_SCORE15 | Corrected the imported distilled-water unit, added pH 7.0, grounded Peptone and Beef extract to complex ontology terms, kept water grounded to CHEBI, and added JCM's default autoclaving instruction. |