Overview
- External ontology
- TOGO:M691 — Flexibacter Canadensis Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M691 Original source: JCM - JCM_M672 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=672
Composition
flowchart LR
CultureMech_010097["flexibacter_canadensis_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010097 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010097 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010097 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_Thiamine_HCl["Thiamine・HCl"]
CultureMech_010097 --> ing3_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing3_Thiamine_HCl -.-> CHEBI_49105
ing4_Vitamin_B12["Vitamin B12"]
CultureMech_010097 --> ing4_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing4_Vitamin_B12 -.-> CHEBI_176843
ing5_KNO3["KNO3"]
CultureMech_010097 --> ing5_KNO3
CHEBI_63043["CHEBI:63043"]
ing5_KNO3 -.-> CHEBI_63043
ing6_Tris_hydroxymethyl_aminomethane["Tris(hydroxymethyl)aminomethane"]
CultureMech_010097 --> ing6_Tris_hydroxymethyl_aminomethane
CHEBI_9754["CHEBI:9754"]
ing6_Tris_hydroxymethyl_aminomethane -.-> CHEBI_9754
ing7_Sodium_glycerophosphate["Sodium glycerophosphate"]
CultureMech_010097 --> ing7_Sodium_glycerophosphate
ing8_Glucose["Glucose"]
CultureMech_010097 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010097 --> ing9_Casamino_acids__BD_Difco_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 5 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 6 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Tris(hydroxymethyl)aminomethane
C4H11NO3 121.136 g/mol
|
1 G_PER_L |
CHEBI:9754
tris
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 8 | Sodium glycerophosphate | 0.1 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Organic compound | |
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 10 | Casamino acids (BD-Difco) | 1 G_PER_L | Properties: Undefined component |
Solutions
Trace element solution SL--10 (see Medium [M433])
Dose: 1 G_PER_L
Cross-reference to Medium M433
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.536968Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M691 |
| 2026-01-28T19:48:42.219378Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:11.152431+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:11.274016Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010097 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.202985+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Flexibacter Canadensis Medium" to "flexibacter_canadensis_medium" for consistent matching |
| 2026-05-16T09:03:20.084632+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:43.594020+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.350000+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |