pyg_medium_f (CultureMech:010099)

Overview

External ontology

TOGO:M693 — PYG Medium (F)

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M693 Original source: JCM - JCM_M674 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=674

Composition

flowchart LR
    CultureMech_010099["pyg_medium_f"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010099 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010099 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_010099 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Trypticase_peptone["Trypticase peptone"]
    CultureMech_010099 --> ing3_Trypticase_peptone
    ing4_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010099 --> ing4_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing5_K2HPO4["K2HPO4"]
    CultureMech_010099 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010099 --> ing6_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_7H2O -.-> CHEBI_31795
    ing7_NaCl["NaCl"]
    CultureMech_010099 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010099 --> ing8_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_2H2O -.-> CHEBI_86158
    ing9_KH2PO4["KH2PO4"]
    CultureMech_010099 --> ing9_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing9_KH2PO4 -.-> CHEBI_63036
    ing10__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010099 --> ing10__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing10__NH4_2SO4 -.-> CHEBI_62946

Ingredients (11)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1050.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 3 duplicates: 850.0, 100.0, 100.0]
2	Yeast extract	5 G_PER_L		Role: Nutrient source; Properties: Undefined component
3	Glucose C6H12O6 180.156 g/mol	10 G_PER_L	CHEBI:17234 glucose	Role: Carbon source; Properties: Simple component, Organic compound, Defined component
4	Trypticase peptone	10 G_PER_L		Properties: Undefined component
5	L--Cysteine・HCl・H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.3 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Properties: Defined component, Simple component, Organic compound
6	K2HPO4 HO4P.2K 174.174 g/mol	0.6 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
7	MgSO4・7H2O 7H2O.Mg.O4S 246.473 g/mol	0.12 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8	NaCl Cl.Na 58.443 g/mol	1.2 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.12 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10	KH2PO4 H2O4P.K 136.084 g/mol	0.6 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
11	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.2 G_PER_L	CHEBI:62946 ammonium sulfate	Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component

Solutions

8% (w/v) Na2CO3 solution

Dose: 2.5 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

0.1% (w/v) Sodium resazurin solution

Dose: 1 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

S I solution (see below)

Dose: 75 G_PER_L

S II solution (see below)

Dose: 75 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:55.541714Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M693
2026-01-28T19:48:21.820076Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:36.453963+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 4 solution(s) from ingredients
2026-03-13T03:09:05.863083Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 2 duplicate ingredient(s)
2026-03-13T06:20:11.295715Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:010099
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:12.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.224801+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "PYG Medium (F)" to "pyg_medium_f" for consistent matching
2026-05-16T09:03:20.108789+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:43.619242+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:32.375605+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.353218+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.