Overview
- External ontology
- TOGO:M696 — Brucella Blood Agar With Hemin Menadione
- Applications
-
- Microbial cultivation
- Notes
- TOGO M696 mirrors JCM Medium 677: 43.0 g Brucella agar (BD-BBL), 50.0 ml horse blood, 10.0 ml hemin solution, 10.0 ml menadione solution, and 1.0 L distilled water.
Composition
flowchart LR
CultureMech_010102["brucella_blood_agar_with_hemin_menadione"]
ing0_Brucella_agar__BD_BBL_["Brucella agar (BD-BBL)"]
CultureMech_010102 --> ing0_Brucella_agar__BD_BBL_
ing1_Horse_blood["Horse blood"]
CultureMech_010102 --> ing1_Horse_blood
ing2_Distilled_water["Distilled water"]
CultureMech_010102 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brucella agar (BD-BBL) | 43.0 G_PER_L | JCM Medium 677 lists 43.0 g Brucella agar (BD-BBL). | |
| 2 | Horse blood | 50.0 ML_PER_L | JCM Medium 677 lists 50.0 ml horse blood. | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 677 lists 1000 ml distilled water. |
Preparation
- Suspend 43.0 g Brucella agar (BD-BBL) in 1.0 L distilled water.
- Add 50.0 ml/L horse blood, 10.0 ml/L hemin solution, and 10.0 ml/L menadione solution.
- Autoclave at 121 C for 15 min unless otherwise stated.
Solutions
Hemin solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Hemin | 0.5 G_PER_L | |
| 1 N NaOH | 10.0 ML_PER_L |
JCM Medium 677 lists 10.0 ml/L Hemin solution from JCM Medium 469.
Menadione solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Menadione | 0.05 G_PER_L | |
| Ethanol | 10.0 ML_PER_L |
JCM Medium 677 lists 10.0 ml/L Menadione solution from JCM Medium 469.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.549212Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M696 |
| 2026-01-28T19:48:40.576917Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:08.390129+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:11.322760Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010102 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.252553+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brucella Blood Agar With Hemin Menadione" to "brucella_blood_agar_with_hemin_menadione" for consistent matching |
| 2026-05-16T09:03:20.142429+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:43.652457+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_official_simple_score20.py | RESOLVED_OFFICIAL_SIMPLE_SCORE20 | TOGO M696 mirrors JCM Medium 677: 43.0 g Brucella agar (BD-BBL), 50.0 ml horse blood, 10.0 ml hemin solution, 10.0 ml menadione solution, and 1.0 L distilled water. |