Overview
- External ontology
- TOGO:M700 — Pfennig's Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M700 Original source: JCM - JCM_M681 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=681
Composition
flowchart LR
CultureMech_010108["pfennigs_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010108 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010108 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010108 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_010108 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2HPO4["K2HPO4"]
CultureMech_010108 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KCl["KCl"]
CultureMech_010108 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2S_9H2O["Na2S・9H2O"]
CultureMech_010108 --> ing6_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_9H2O -.-> CHEBI_76209
ing7_NaHCO3["NaHCO3"]
CultureMech_010108 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Vitamin_B12["Vitamin B12"]
CultureMech_010108 --> ing8_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing8_Vitamin_B12 -.-> CHEBI_176843
ing9_Acetate__final_concentration_["Acetate (final concentration)"]
CultureMech_010108 --> ing9_Acetate__final_concentration_
ing10_Thiosulfate__final_concentration_["Thiosulfate (final concentration)"]
CultureMech_010108 --> ing10_Thiosulfate__final_concentration_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.34 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.25 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.34 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 7 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 9 | Vitamin B12 | 0.02 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Simple component, Organic compound, Defined component |
| 10 | Acetate (final concentration) | 2 MILLIMOLAR | Role: Carbon source; Properties: Defined component, Organic compound, Simple component | |
| 11 | Thiosulfate (final concentration) | 10 MILLIMOLAR | Role: Growth factor; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Trace element solution SL--10 (see Medium [M433])
Dose: 0.5 G_PER_L
Cross-reference to Medium M433
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.560279Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M700 |
| 2026-01-28T19:48:30.907211Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:51.412435+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:11.378799Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010108 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.308010+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pfennig's Medium" to "pfennigs_medium" for consistent matching |
| 2026-05-16T09:03:20.204836+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:43.716167+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.459640+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.392639+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |