Overview
- External ontology
- TOGO:M701 — Methanofollis Ethanolicus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M701 Original source: JCM - JCM_M682 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=682
Composition
flowchart LR
CultureMech_010109["methanofollis_ethanolicus_medium"]
ing0_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
CultureMech_010109 --> ing0_L__cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing0_L__cysteine_HCl_H2O -.-> CHEBI_91248
ing1_N2["N2"]
CultureMech_010109 --> ing1_N2
CHEBI_17997["CHEBI:17997"]
ing1_N2 -.-> CHEBI_17997
ing2_Distilled_water["Distilled water"]
CultureMech_010109 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010109 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010109 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_010109 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_010109 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010109 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_NaHCO3["NaHCO3"]
CultureMech_010109 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010109 --> ing9_Yeast_extract__BD_Difco_
ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010109 --> ing10_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing10_Carbon_dioxide_gas -.-> CHEBI_16526
ing11_Nitrogen_gas["Nitrogen gas"]
CultureMech_010109 --> ing11_Nitrogen_gas
ing11_Nitrogen_gas -.-> CHEBI_17997
ing12_Ethanol["Ethanol"]
CultureMech_010109 --> ing12_Ethanol
CHEBI_16236["CHEBI:16236"]
ing12_Ethanol -.-> CHEBI_16236
ing13_Biotin["Biotin"]
CultureMech_010109 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_010109 --> ing14_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing14_p__Aminobenzoic_acid -.-> CHEBI_30753
ing15_Thiamine_HCl["Thiamine・HCl"]
CultureMech_010109 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
ing16_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_010109 --> ing16_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_HCl -.-> CHEBI_30961
ing17_Folic_acid["Folic acid"]
CultureMech_010109 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_010109 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_Riboflavin["Riboflavin"]
CultureMech_010109 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_010109 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Lipoic_acid["Lipoic acid"]
CultureMech_010109 --> ing21_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing21_Lipoic_acid -.-> CHEBI_16494
ing22_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_010109 --> ing22_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_DL_Calcium_pantothenate -.-> CHEBI_31345
ing23_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010109 --> ing23_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_2H2O -.-> CHEBI_75213
ing24_H3BO3["H3BO3"]
CultureMech_010109 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010109 --> ing25_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing25_MnCl2_4H2O -.-> CHEBI_86368
ing26_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010109 --> ing26_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing26_CoCl2_6H2O -.-> CHEBI_35696
ing27_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010109 --> ing27_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing27_NiCl2_6H2O -.-> CHEBI_34887
ing28_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_010109 --> ing28_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing28_CuCl2_2H2O -.-> CHEBI_86318
ing29_ZnCl2["ZnCl2"]
CultureMech_010109 --> ing29_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing29_ZnCl2 -.-> CHEBI_49976
CultureMech_010109 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Solution |
| 2 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1002.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 900.0, 100.0, 1.0, 1.0] |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.54 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 | Yeast extract (BD-Difco) | 0.1 G_PER_L | Properties: Undefined component | |
| 11 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 12 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Ethanol
C2H6O 46.069 g/mol
|
0.46 G_PER_L |
CHEBI:16236
ethanol
|
Role: Solvating media; Properties: Organic compound, Defined component, Simple component |
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
4.9 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
2.7 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
6.7 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
4.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
8.8 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
7.5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
2.5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
4.1 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 23 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
9.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.2 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.13 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.001 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.14 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.27 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.003 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.002 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
AlCl3
AlCl3 133.341 g/mol
|
0.01 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Na2S・9H2O solutions
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
Solution A
Dose: 900 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Trace vitamins solution (see below)
Dose: 2 G_PER_L
Trace element solution (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.562035Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M701 |
| 2026-01-28T19:48:26.465217Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:43.942898+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:14.126807Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:11.393694Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010109 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.325217+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanofollis Ethanolicus Medium" to "methanofollis_ethanolicus_medium" for consistent matching |
| 2026-05-16T09:03:20.221310+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.732770+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.834897+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:51.396492+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |