Overview
- External ontology
- TOGO:M705 — Alicyclobacillus Ferrooxydans Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M705 Original source: JCM - JCM_M685-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=685
Composition
flowchart LR
CultureMech_010112["alicyclobacillus_ferrooxydans_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010112 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010112 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_010112 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_010112 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KCl["KCl"]
CultureMech_010112 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_010112 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_K2S4O6["K2S4O6"]
CultureMech_010112 --> ing6_K2S4O6
CHEBI_86466["CHEBI:86466"]
ing6_K2S4O6 -.-> CHEBI_86466
ing7_H2SO4["H2SO4"]
CultureMech_010112 --> ing7_H2SO4
CHEBI_26836["CHEBI:26836"]
ing7_H2SO4 -.-> CHEBI_26836
ing8_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010112 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.5 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
K2S4O6
2K.O6S4 302.458 g/mol
|
0.15 G_PER_L |
CHEBI:86466
potassium tetrathionate
|
Properties: Defined component, Inorganic compound, Simple component |
| 8 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 9 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
20 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Solution A
Dose: 0.5 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 70 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Gelrite (gellan gum) solution
Dose: 0.5 G_PER_L
Gelrite (gellan gum) solution
Dose: 0.5 G_PER_L
Role: Solidifying component; Properties: Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.570065Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M705 |
| 2026-01-28T19:48:40.338325Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:05:07.989629+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:39.706594Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.422600Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010112 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.353913+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alicyclobacillus Ferrooxydans Medium" to "alicyclobacillus_ferrooxydans_medium" for consistent matching |
| 2026-05-16T09:03:20.255031+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.765726+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.499243+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.413076+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |