methanol_utilizing_bacteria_medium_b

CultureMech:010117 bacterial DEFINED LIQUID
Original name: Methanol-Utilizing Bacteria Medium B

Overview

External ontology
TOGO:M70 — Methanol-Utilizing Bacteria Medium B
Applications
  • Microbial cultivation
pH
7.1
Notes
TOGO M70 mirrors JCM Medium 79: 10.0 ml methanol, defined salts, 0.4 mg Thiamine HCl, and distilled water per liter; adjust pH to 7.1.

Composition

flowchart LR
    CultureMech_010117["methanol_utilizing_bacteria_medium_b"]
    ing0_Methanol["Methanol"]
    CultureMech_010117 --> ing0_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing0_Methanol -.-> CHEBI_17790
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010117 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_KH2PO4["KH2PO4"]
    CultureMech_010117 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_010117 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_010117 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Ferric_citrate["Ferric citrate"]
    CultureMech_010117 --> ing5_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing5_Ferric_citrate -.-> CHEBI_144421
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_010117 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_010117 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_010117 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_010117 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_Thiamine_HCl["Thiamine HCl"]
    CultureMech_010117 --> ing10_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing10_Thiamine_HCl -.-> CHEBI_49105
    ing11_Distilled_water["Distilled water"]
    CultureMech_010117 --> ing11_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Distilled_water -.-> CHEBI_15377

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Methanol
CH4O 32.042 g/mol
10.0 ML_PER_L CHEBI:17790
methanol
 JCM Medium 79 lists 10.0 ML_PER_L Methanol per liter.
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3.0 G_PER_L CHEBI:62946
ammonium sulfate
 JCM Medium 79 lists 3.0 G_PER_L (NH4)2SO4 per liter.
3 KH2PO4
H2O4P.K 136.084 g/mol
1.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 JCM Medium 79 lists 1.4 G_PER_L KH2PO4 per liter.
4 Na2HPO4
HO4P.2Na 141.958 g/mol
3.0 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 JCM Medium 79 lists 3.0 G_PER_L Na2HPO4 per liter.
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 JCM Medium 79 lists 0.2 G_PER_L MgSO4 x 7 H2O per liter.
6 Ferric citrate
C6H5FeO7 244.944 g/mol
30.0 MG_PER_L CHEBI:144421
iron(III) citrate
 JCM Medium 79 lists 30.0 MG_PER_L Ferric citrate per liter.
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
30.0 MG_PER_L CHEBI:86158
calcium chloride dihydrate
 JCM Medium 79 lists 30.0 MG_PER_L CaCl2 x 2 H2O per liter.
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
5.0 MG_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 JCM Medium 79 lists 5.0 MG_PER_L MnCl2 x 4 H2O per liter.
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
5.0 MG_PER_L CHEBI:32312
zinc sulfate heptahydrate
 JCM Medium 79 lists 5.0 MG_PER_L ZnSO4 x 7 H2O per liter.
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.5 MG_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
 JCM Medium 79 lists 0.5 MG_PER_L CuSO4 x 5 H2O per liter.
11 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.4 MG_PER_L CHEBI:49105
thiamine hydrochloride
 JCM Medium 79 lists 0.4 mg/L Thiamine HCl and notes that it can be replaced by 0.2 g yeast extract.
12 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 79 lists 1.0 L Distilled water per liter.

Preparation

  1. Dissolve the Medium B components in 1.0 L distilled water.
  2. Adjust pH to 7.1.
  3. Autoclave at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.558497Z togo-import Imported from TOGO Medium Source: JCM, ID: M70
2026-01-28T19:48:17.102925Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:11.459611Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010117
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.391141+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanol-Utilizing Bacteria Medium B" to "methanol_utilizing_bacteria_medium_b" for consistent matching
2026-06-05T07:40:43.798813+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:32.526873+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.427110+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-06T00:00:00-07:00 repair_methanol_utilizing_score40.py RESOLVED_METHANOL_UTILIZING_SCORE40_GRAPH TOGO M70 mirrors JCM Medium 79: 10.0 ml methanol, defined salts, 0.4 mg Thiamine HCl, and distilled water per liter; adjust pH to 7.1.