aerobic_sulfolobales_medium_without_sulfur

CultureMech:010120 archaea COMPLEX LIQUID
Original name: Aerobic Sulfolobales Medium Without Sulfur

Overview

External ontology
TOGO:M713 — Aerobic Sulfolobales Medium Without Sulfur
Applications
  • Microbial cultivation
pH
2.5
Notes
TOGO M713/JCM Medium 693 prepares Aerobic Sulfolobales Medium from TOGO M711/JCM Medium 691 and omits the elemental sulfur.

Composition

flowchart LR
    CultureMech_010120["aerobic_sulfolobales_medium_without_sulfur"]
    ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010120 --> ing0_Yeast_extract__BD_Difco_
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_010120 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_010120 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_010120 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_010120 --> ing4_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing4_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_010120 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_010120 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_010120 --> ing7_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing7_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing8__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010120 --> ing8__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing8__NH4_2SO4 -.-> CHEBI_62946
    ing9_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_010120 --> ing9_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing9_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing10_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_010120 --> ing10_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing10_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_010120 --> ing11_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing12_H2SO4["H2SO4"]
    CultureMech_010120 --> ing12_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing12_H2SO4 -.-> CHEBI_26836

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Yeast extract (BD-Difco) 0.5 G_PER_L TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
4 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
5 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.00003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
6 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0018 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.00022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
8 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.00005 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
9 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
10 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.00001 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
11 VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
0.00003 G_PER_L CHEBI:87020
vanadyl sulfate hydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
12 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0045 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.
13 H2SO4
H2O4S 98.079 g/mol
variable G_PER_L CHEBI:26836
sulfuric acid
 TOGO M711 and JCM Medium 691 list this component for Aerobic Sulfolobales Medium; TOGO M713 omits sulfur.

Preparation

  1. Prepare the TOGO M711 salt-base composition without elemental sulfur for the TOGO M713 sulfur-free variant.
  2. Autoclave salt base and yeast extract separately and combine after cooling.
  3. Adjust the complete medium to pH 2.5.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.582985Z togo-import Imported from TOGO Medium Source: JCM, ID: M713
2026-03-09T08:04:49.963989+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:11.478322Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010120
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T19:46:30.409500+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Aerobic Sulfolobales Medium Without Sulfur" to "aerobic_sulfolobales_medium_without_sulfur" for consistent matching
2026-05-16T09:03:20.301892+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-09-06T00:00:00-07:00 repair_archaea_togo_score40.py RESOLVED_ARCHAEA_TOGO_SCORE40_GRAPH TOGO M713/JCM Medium 693 prepares Aerobic Sulfolobales Medium from TOGO M711/JCM Medium 691 and omits the elemental sulfur.