spirochaeta_smaeagdinae_medium

CultureMech:010121 bacterial COMPLEX LIQUID
Original name: Spirochaeta Smaeagdinae Medium

Overview

External ontology
TOGO:M714 — Spirochaeta Smaeagdinae Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M714 Original source: JCM - JCM_M694 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=694

Composition

flowchart LR
    CultureMech_010121["spirochaeta_smaeagdinae_medium"]
    ing0_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010121 --> ing0_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing0_Carbon_dioxide_gas -.-> CHEBI_16526
    ing1_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010121 --> ing1_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing1_Nitrogen_gas -.-> CHEBI_17997
    ing2_Distilled_water["Distilled water"]
    CultureMech_010121 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_010121 --> ing3_Yeast_extract
    ing4_NaCl["NaCl"]
    CultureMech_010121 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_KH2PO4["KH2PO4"]
    CultureMech_010121 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_010121 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_K2HPO4["K2HPO4"]
    CultureMech_010121 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_Resazurin["Resazurin"]
    CultureMech_010121 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_KCl["KCl"]
    CultureMech_010121 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010121 --> ing10_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing11_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010121 --> ing11_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_2H2O -.-> CHEBI_86158
    ing12_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010121 --> ing12_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing12_MgCl2_6H2O -.-> CHEBI_86345

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
2 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
3 Distilled water
H2O 18.015 g/mol
950.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 900.0, 50.0]
4 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
5 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 KH2PO4
H2O4P.K 136.084 g/mol
0.33 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
8 K2HPO4
HO4P.2K 174.174 g/mol
0.33 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
9 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
10 KCl
Cl.K 74.551 g/mol
0.2 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
11 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
12 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
13 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component

Solutions

Solution A

Dose: 910 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 50 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

8% NaHCO3 solution*

Dose: 50 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

5% Na2S・9H2O solution

Dose: 4 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.583706Z togo-import Imported from TOGO Medium Source: JCM, ID: M714
2026-01-28T19:48:30.844551Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:51.311290+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T03:09:22.655895Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:11.486051Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010121
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.416686+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Spirochaeta Smaeagdinae Medium" to "spirochaeta_smaeagdinae_medium" for consistent matching
2026-05-16T09:03:20.310026+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:43.819041+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.435027+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.