mjy_medium_for_pyrolinea_marinus

Overview

External ontology

TOGO:M722 — MJY Medium For Pyrolinea Marinus

Applications

• Microbial cultivation

pH range

{'min': 6.0, 'max': 6.5}

Notes

MediaDive J700 and TOGO M721 describe JCM Medium 700 MJY Medium for Pyrolinea marinus as 1.0 L MJ(-N) synthetic seawater supplemented with 1.0 g/L Yeast extract, 0.25 g/L NH4Cl, and 0.5 g/L NaNO3; after anaerobic autoclaving, 5.0 ml/L 8% NaHCO3 and 10.0 ml/L 5% Na2S x 9 H2O solutions are added and the final pH is checked at 6.0-6.5. TOGO M722 additionally adds 20.0 ml/L Rumen fluid, clarified from TOGO M258/JCM Medium 266.

Composition

flowchart LR
    CultureMech_010130["mjy_medium_for_pyrolinea_marinus"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_010130 --> ing0_Yeast_extract
    ing1_NH4Cl["NH4Cl"]
    CultureMech_010130 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_NaNO3["NaNO3"]
    CultureMech_010130 --> ing2_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing2_NaNO3 -.-> CHEBI_63005

Ingredients (3)

Preparation

1. Mix 1.0 L MJ(-N) synthetic seawater, NH4Cl, NaNO3, and Yeast extract thoroughly; distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave.
2. After cooling, add 5.0 ml/L 8% NaHCO3 solution and 10.0 ml/L 5% Na2S x 9 H2O solution that were filter-sterilized or autoclaved and stored anaerobically.
3. Pressurize the culture vessels to 200 kPa N2-CO2 (4:1, v/v) and check the final pH at 6.0-6.5.
4. MJ(-N) synthetic seawater can be replaced with a commercial synthetic seawater such as Jamarin S. For strain JCM 15506, inoculate with a 5-10% inoculum.

Solutions

Target organisms

No target organisms are recorded.

Provenance