Overview
- External ontology
- TOGO:M726 — Anoxybacillus Tunisiense Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M726 Original source: JCM - JCM_M704 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=704
Composition
flowchart LR
CultureMech_010134["anoxybacillus_tunisiense_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010134 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010134 --> ing1_Yeast_extract__BD_Difco_
ing2_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010134 --> ing2_Tryptone__BD_Difco_
ing3_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010134 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_010134 --> ing4_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
ing5_CaSO4_2H2O["CaSO4・2H2O"]
CultureMech_010134 --> ing5_CaSO4_2H2O
CHEBI_32583["CHEBI:32583"]
ing5_CaSO4_2H2O -.-> CHEBI_32583
ing6_KH2PO4["KH2PO4"]
CultureMech_010134 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_Na2HPO4_2H2O["Na2HPO4・2H2O"]
CultureMech_010134 --> ing7_Na2HPO4_2H2O
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4_2H2O -.-> CHEBI_34683
ing8_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010134 --> ing8_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_2H2O -.-> CHEBI_75213
ing9_H3BO3["H3BO3"]
CultureMech_010134 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010134 --> ing10_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_4H2O -.-> CHEBI_86368
ing11_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010134 --> ing11_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing11_CoCl2_6H2O -.-> CHEBI_35696
ing12_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010134 --> ing12_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_6H2O -.-> CHEBI_34887
ing13_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_010134 --> ing13_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_2H2O -.-> CHEBI_86318
ing14_ZnCl2["ZnCl2"]
CultureMech_010134 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_010134 --> ing15_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_4H2O -.-> CHEBI_86249
ing16_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_010134 --> ing16_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing16_FeCl3_6H2O -.-> CHEBI_86254
ing17_Sodium_citrate_2H2O["Sodium citrate・2H2O"]
CultureMech_010134 --> ing17_Sodium_citrate_2H2O
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1873.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 880.0, 990.0, 1.0, 1.0, 1.0] |
| 2 | Yeast extract (BD-Difco) | 2.5 G_PER_L | Properties: Undefined component | |
| 3 | Tryptone (BD-Difco) | 2.5 G_PER_L | Properties: Undefined component | |
| 4 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
14.120000000000001 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 1.32, 12.8] |
| 6 |
CaSO4・2H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.4 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
5.44 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2HPO4・2H2O
HO4P.2Na 141.958 g/mol
|
21.4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.02 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.3 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.2 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
2.7 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 | Sodium citrate・2H2O | 2.94 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component |
Solutions
Base solution A (see below)
Dose: 100 G_PER_L
Base solution B (see below)
Dose: 20 G_PER_L
Trace elements (see below)
Dose: 5 G_PER_L
Fe citrate solution (see below)
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.606794Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M726 |
| 2026-01-28T19:48:25.574779Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:42.338831+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:12.359004Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:11.585033Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010134 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.515961+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anoxybacillus Tunisiense Medium" to "anoxybacillus_tunisiense_medium" for consistent matching |
| 2026-05-16T09:03:20.419112+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:43.936444+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.477826+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |