Overview
- External ontology
- TOGO:M733 — AG Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M733 Original source: JCM - JCM_M710 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=710
Composition
flowchart LR
CultureMech_010142["ag_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010142 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_D__Glucose["D--Glucose"]
CultureMech_010142 --> ing1_D__Glucose
ing2_CaCO3["CaCO3"]
CultureMech_010142 --> ing2_CaCO3
CHEBI_3311["CHEBI:3311"]
ing2_CaCO3 -.-> CHEBI_3311
ing3_Glycerol["Glycerol"]
CultureMech_010142 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_Agar["Agar"]
CultureMech_010142 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010142 --> ing5_Yeast_extract__BD_Difco_
ing6_Malt_extract["Malt extract"]
CultureMech_010142 --> ing6_Malt_extract
ing7_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_010142 --> ing7_Bacto_peptone__BD_Difco_
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | D--Glucose | 10 G_PER_L | Role: Carbon source; Properties: Defined component, Organic compound, Simple component | |
| 3 |
CaCO3
CO3.Ca 100.086 g/mol
|
7 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Protective agent, Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
15 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 7 | Malt extract | 2 G_PER_L | Properties: Undefined component | |
| 8 | Bacto peptone (BD-Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.622150Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M733 |
| 2026-01-28T19:48:12.817720Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:11.648188Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010142 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.579488+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AG Medium" to "ag_medium" for consistent matching |
| 2026-06-05T07:40:44.014579+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.885701+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |