alkaline_yeast_extract_malt_extract_agar

CultureMech:010146 bacterial COMPLEX SOLID_AGAR
Original name: Alkaline Yeast Extract-Malt Extract Agar

Overview

External ontology
TOGO:M73 — Alkaline Yeast Extract-Malt Extract Agar
Applications
  • Microbial cultivation
pH
10.0
Notes
TOGO M73 mirrors JCM Medium 82: Alkaline Yeast Extract-Malt Extract Agar uses JCM Medium 43 ISP-2 agar adjusted to pH 10.0 with sterilized 10% Na2CO3 solution after autoclaving.

Composition

flowchart LR
    CultureMech_010146["alkaline_yeast_extract_malt_extract_agar"]
    ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010146 --> ing0_Yeast_extract__BD_Difco_
    ing1_Malt_extract__BD_Difco_["Malt extract (BD-Difco)"]
    CultureMech_010146 --> ing1_Malt_extract__BD_Difco_
    ing2_Glucose["Glucose"]
    CultureMech_010146 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Agar["Agar"]
    CultureMech_010146 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_010146 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Yeast extract (BD-Difco) 4.0 G_PER_L FOODON:03315426
yeast extract
 JCM Medium 43 prints this component.
2 Malt extract (BD-Difco) 10.0 G_PER_L FOODON:03301056
malt extract
 JCM Medium 43 prints this component.
3 Glucose
C6H12O6 180.156 g/mol
4.0 G_PER_L CHEBI:17234
glucose
 JCM Medium 43 prints this component.
4 Agar 20.0 G_PER_L CHEBI:2509
agar
 JCM Medium 43 prints this component.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 43 lists 1.0 l distilled water.

Preparation

  1. Prepare Yeast extract-malt extract agar (ISP-2) from JCM Medium 43.
  2. Autoclave the ISP-2 agar base at 121 C for 15 min.
  3. After autoclaving, adjust pH to 10.0 with sterilized 10% Na2CO3 solution.

Solutions

10% Na2CO3 solution

Dose: variable VARIABLE

Stock composition
IngredientConcentrationNotes
Na2CO3 10.0 PERCENT_W_V JCM Medium 82 prints this component in its solution subrecipe.
JCM Medium 82 adjusts the autoclaved ISP-2 agar base to pH 10.0 with sterilized 10% Na2CO3 solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.616682Z togo-import Imported from TOGO Medium Source: JCM, ID: M73
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:04:19.292501+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:11.680143Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010146
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T19:46:30.611023+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Alkaline Yeast Extract-Malt Extract Agar" to "alkaline_yeast_extract_malt_extract_agar" for consistent matching
2026-05-16T09:03:20.517804+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-09-06T00:00:00-07:00 repair_jcm_more_wrappers_score40.py RESOLVED_JCM_MORE_SCORE40_GRAPH TOGO M73 mirrors JCM Medium 82: Alkaline Yeast Extract-Malt Extract Agar uses JCM Medium 43 ISP-2 agar adjusted to pH 10.0 with sterilized 10% Na2CO3 solution after autoclaving.