Overview
- External ontology
- TOGO:M745 — CYS Medium For Strain SS
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M745 Original source: JCM - JCM_M722 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=722
Composition
flowchart LR
CultureMech_010150["cys_medium_for_strain_ss"]
ing0_Distilled_water["Distilled water"]
CultureMech_010150 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010150 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Soluble_starch__Sigma_["Soluble starch (Sigma)"]
CultureMech_010150 --> ing2_Soluble_starch__Sigma_
ing3_Dried_yeast_extract_S__Wako_["Dried yeast extract S (Wako)"]
CultureMech_010150 --> ing3_Dried_yeast_extract_S__Wako_
ing4_Hy__Case_Amino__Sigma_["Hy--Case Amino (Sigma)"]
CultureMech_010150 --> ing4_Hy__Case_Amino__Sigma_
ing5_NaOH["NaOH"]
CultureMech_010150 --> ing5_NaOH
CHEBI_32145["CHEBI:32145"]
ing5_NaOH -.-> CHEBI_32145
ing6_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010150 --> ing6_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_2H2O -.-> CHEBI_86158
ing7_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010150 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010150 --> ing8_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_6H2O -.-> CHEBI_86345
ing9_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010150 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010150 --> ing10_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_4H2O -.-> CHEBI_86368
ing11_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010150 --> ing11_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing11_CoCl2_6H2O -.-> CHEBI_35696
ing12_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010150 --> ing12_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_6H2O -.-> CHEBI_34887
ing13_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010150 --> ing13_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_7H2O -.-> CHEBI_32312
ing14_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010150 --> ing14_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_5H2O -.-> CHEBI_31440
ing15_VOSO4_xH2O["VOSO4・xH2O"]
CultureMech_010150 --> ing15_VOSO4_xH2O
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Soluble starch (Sigma) | 1 G_PER_L | Role: Carbon source; Properties: Undefined component | |
| 4 | Dried yeast extract S (Wako) | 2 G_PER_L | Properties: Undefined component | |
| 5 | Hy--Case Amino (Sigma) | 3 G_PER_L | Properties: Undefined component | |
| 6 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 7 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
50 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.12 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.25 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
50 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
80 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
6 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.5 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 | VOSO4・xH2O | 20 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace mineral solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.635835Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M745 |
| 2026-01-28T19:48:20.893425Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:34.664752+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:04.218635Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.703473Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010150 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.633557+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CYS Medium For Strain SS" to "cys_medium_for_strain_ss" for consistent matching |
| 2026-05-16T09:03:20.537487+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.069262+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-02T07:37:52.089672+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |