Overview
- External ontology
- TOGO:M751 — Desulfosporosinus Acidiphilus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M751 Original source: JCM - JCM_M728 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=728
Composition
flowchart LR
CultureMech_010157["desulfosporosinus_acidiphilus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010157 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010157 --> ing1_Yeast_extract
ing2_K2SO4["K2SO4"]
CultureMech_010157 --> ing2_K2SO4
CHEBI_32036["CHEBI:32036"]
ing2_K2SO4 -.-> CHEBI_32036
ing3_H2SO4["H2SO4"]
CultureMech_010157 --> ing3_H2SO4
CHEBI_26836["CHEBI:26836"]
ing3_H2SO4 -.-> CHEBI_26836
ing4_N2["N2"]
CultureMech_010157 --> ing4_N2
CHEBI_17997["CHEBI:17997"]
ing4_N2 -.-> CHEBI_17997
ing5_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010157 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_K2HPO4["K2HPO4"]
CultureMech_010157 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_KCl["KCl"]
CultureMech_010157 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_010157 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_Na2SO4["Na2SO4"]
CultureMech_010157 --> ing9_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing9_Na2SO4 -.-> CHEBI_32149
ing10_Ca_NO3_2_4H2O["Ca(NO3)2・4H2O"]
CultureMech_010157 --> ing10_Ca_NO3_2_4H2O
CHEBI_86159["CHEBI:86159"]
ing10_Ca_NO3_2_4H2O -.-> CHEBI_86159
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
940.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 939.0, 1.0] |
| 2 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
K2SO4
2K.O4S 174.259 g/mol
|
0.87 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 5 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 6 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
2.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
22.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.3 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
Ca(NO3)2・4H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.7 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
Solutions
HBS solution (see below)
Dose: 20 G_PER_L
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
Ni-Se-W solution (see Medium [M236])
Dose: 10 G_PER_L
Cross-reference to Medium M236
1 M ZnSO4 solution
Dose: 7 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
10 mM FeSO4 solution (pH 2.0)
Dose: 10 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
1 M Glycerol solution
Dose: 4 G_PER_L
Role: Carbon source; Properties: Solution, Organic compound, Defined component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.645907Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M751 |
| 2026-01-28T19:48:17.624456Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:29.170564+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:08:58.009339Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.745056Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010157 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.676118+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfosporosinus Acidiphilus Medium" to "desulfosporosinus_acidiphilus_medium" for consistent matching |
| 2026-05-16T09:03:20.578491+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.109974+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.763968+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.532459+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |