Overview
- External ontology
- TOGO:M763 — BM For Tepidanaerobacter Acetoxydans
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 738 / TOGO M763 lists a defined anaerobic base with yeast extract, Trace metal solution from TOGO M288, Selenite-tungstate solution from TOGO M431, 8% NaHCO3, 0.1 M glucose, 5% Na2S x 9H2O, Phosphate solution from JCM Medium 737 / TOGO M762, Trace vitamins from TOGO M190, and an N2-CO2 (80:20, v/v) gas phase. The glucose stock replaces the 0.1 M L-sodium lactate stock in JCM Medium 737.
Composition
flowchart LR
CultureMech_010170["bm_for_tepidanaerobacter_acetoxydans"]
ing0_Distilled_water["Distilled water"]
CultureMech_010170 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010170 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_010170 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_x_2H2O["CaCl2 x 2H2O"]
CultureMech_010170 --> ing3_CaCl2_x_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_010170 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_010170 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_x_6H2O["MgCl2 x 6H2O"]
CultureMech_010170 --> ing6_MgCl2_x_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6H2O -.-> CHEBI_86345
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010170 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_010170 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
830.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 738 / TOGO M763 lists 830 ml distilled water before stock additions. |
| 2 | Yeast extract | 0.2 G_PER_L |
FOODON:03315426
yeast extract
|
JCM Medium 738 / TOGO M763 lists 0.2 g/L Yeast extract. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.85 G_PER_L |
CHEBI:26710
sodium chloride
|
JCM Medium 738 / TOGO M763 lists 0.85 g/L NaCl. |
| 4 |
CaCl2 x 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
JCM Medium 738 / TOGO M763 lists 0.1 g/L CaCl2 x 2H2O. |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.85 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 738 / TOGO M763 lists 0.85 g/L NH4Cl. |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
JCM Medium 738 / TOGO M763 lists 0.5 mg/L Resazurin. |
| 7 |
MgCl2 x 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.28 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
JCM Medium 738 / TOGO M763 lists 0.28 g/L MgCl2 x 6H2O. |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 738 / TOGO M763 uses an N2-CO2 (80:20, v/v) gas atmosphere. |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 738 / TOGO M763 uses an N2-CO2 (80:20, v/v) gas atmosphere. |
Preparation
- Mix the base ingredients, Trace metal solution, and Selenite-tungstate solution under an N2-CO2 (80:20, v/v) gas mixture.
- Autoclave the base medium under the same gas mixture in bottles sealed with butyl rubber stoppers.
- After cooling, aseptically and anaerobically add the NaHCO3, glucose, Na2S x 9H2O, Phosphate, and Trace vitamins stocks.
Solutions
Trace metal solution (TOGO Medium M288)
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| HCl (32%) | 10.0 ML_PER_L | JCM Medium 294 / TOGO M288 lists 10.0 ml/L HCl (32%). |
| Distilled water | 1.0 L | JCM Medium 294 / TOGO M288 lists 1.0 L Distilled water. |
| Na2MoO4 x 2H2O | 0.04 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.04 g/L Na2MoO4 x 2H2O. |
| H3BO3 | 0.006 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.006 g/L H3BO3. |
| MnCl2 x 4H2O | 0.1 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.1 g/L MnCl2 x 4H2O. |
| CoCl2 x 6H2O | 0.25 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.25 g/L CoCl2 x 6H2O. |
| NiCl2 x 6H2O | 0.07 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.07 g/L NiCl2 x 6H2O. |
| CuCl2 x 2H2O | 0.002 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.002 g/L CuCl2 x 2H2O. |
| ZnCl2 | 0.07 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.07 g/L ZnCl2. |
| Na2WO4 x 2H2O | 0.006 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.006 g/L Na2WO4 x 2H2O. |
| AlCl3 | 0.025 G_PER_L | JCM Medium 294 / TOGO M288 lists 0.025 g/L AlCl3. |
| FeCl2 x 4H2O | 2.0 G_PER_L | JCM Medium 294 / TOGO M288 lists 2.0 g/L FeCl2 x 4H2O. |
JCM Medium 738 adds 1.0 ml/L Trace metal solution from TOGO M288.
Selenite-tungstate solution (TOGO Medium M431)
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1.0 L | JCM Medium 431 / TOGO M431 lists 1.0 L Distilled water. |
| Na2SeO3 x 5H2O | 6.0 MG_PER_L | JCM Medium 431 / TOGO M431 lists 6.0 mg/L Na2SeO3 x 5H2O. |
| Na2WO4 x 2H2O | 8.0 MG_PER_L | JCM Medium 431 / TOGO M431 lists 8.0 mg/L Na2WO4 x 2H2O. |
| NaOH | 0.4 G_PER_L | JCM Medium 431 / TOGO M431 lists 0.4 g/L NaOH. |
JCM Medium 738 adds 1.0 ml/L Selenite-tungstate solution from TOGO M431.
8% NaHCO3 solution
Dose: 40.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 8.0 PERCENT_W_V | JCM Medium 738 / TOGO M763 lists 8.0 % w/v NaHCO3. |
JCM Medium 738 adds 40.0 ml/L filter-sterilized 8% NaHCO3 solution.
0.1 M glucose solution
Dose: 100.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 0.1 MOLAR | JCM Medium 738 / TOGO M763 lists 0.1 M Glucose. |
JCM Medium 738 adds 100.0 ml/L 0.1 M glucose solution in place of JCM Medium 737's 0.1 M L-sodium lactate solution.
5% Na2S x 9H2O solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 5.0 PERCENT_W_V | JCM Medium 738 / TOGO M763 lists 5.0 % w/v Na2S x 9H2O. |
JCM Medium 738 adds 5.0 ml/L 5% Na2S x 9H2O solution.
Phosphate solution (TOGO Medium M762)
Dose: 25.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1000.0 ML_PER_L | JCM Medium 737 / TOGO M762 lists 1000.0 ml/L Distilled water. |
| KH2PO4 | 4.1 G_PER_L | JCM Medium 737 / TOGO M762 lists 0.41 g KH2PO4 in a 100 ml Phosphate solution; this records the stock as a per-liter concentration. |
| Na2HPO4 | 4.3 G_PER_L | JCM Medium 737 / TOGO M762 lists 0.43 g Na2HPO4 in a 100 ml Phosphate solution; this records the stock as a per-liter concentration. |
JCM Medium 738 adds 25.0 ml/L Phosphate solution from TOGO M762.
Trace vitamins (TOGO Medium M190)
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1.0 L | JCM Medium 197 / TOGO M190 lists 1.0 L Distilled water. |
| Biotin | 2.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 2.0 mg/L Biotin. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 5.0 mg/L p-Aminobenzoic acid. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 5.0 mg/L Thiamine HCl. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 5.0 mg/L Calcium pantothenate. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 10.0 mg/L Pyridoxine HCl. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 2.0 mg/L Folic acid. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 / TOGO M190 lists 0.1 mg/L Vitamin B12. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 5.0 mg/L Nicotinic acid. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 / TOGO M190 lists 5.0 mg/L Lipoic acid. |
JCM Medium 738 adds 1.0 ml/L Trace vitamins from TOGO M190.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.664782Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M763 |
| 2026-01-28T19:48:29.457867Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:48.936040+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T06:20:11.825634Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010170 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.759532+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BM For Tepidanaerobacter Acetoxydans" to "bm_for_tepidanaerobacter_acetoxydans" for consistent matching |
| 2026-05-16T09:03:20.650775+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.195021+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.557429+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m763_tepidanaerobacter_score15.py | RESOLVED_JCM_738_TOGO_M763_SCORE15 | Expanded M763 stock-solution wrappers with TOGO M288, M431, M762, and M190 compositions; corrected liquid additions and resazurin units; grounded source-disclosed components; and linked JCM J738 to the TOGO M763 source duplicate. |