Overview
- External ontology
- TOGO:M766 — Acetobacterium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M766 Original source: JCM - JCM_M741 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=741
Composition
flowchart LR
CultureMech_010173["acetobacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010173 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010173 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_010173 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_010173 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_010173 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_010173 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_010173 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Na2S_9H2O["Na2S・9H2O"]
CultureMech_010173 --> ing7_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_9H2O -.-> CHEBI_76209
ing8_NaHCO3["NaHCO3"]
CultureMech_010173 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Fructose["Fructose"]
CultureMech_010173 --> ing9_Fructose
CHEBI_28645["CHEBI:28645"]
ing9_Fructose -.-> CHEBI_28645
ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_010173 --> ing10_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010173 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_010173 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
ing13_NaCl["NaCl"]
CultureMech_010173 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010173 --> ing14_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_2H2O -.-> CHEBI_86158
ing15_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010173 --> ing15_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_2H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_010173 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010173 --> ing17_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_7H2O -.-> CHEBI_75836
ing18_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010173 --> ing18_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_6H2O -.-> CHEBI_34887
ing19_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010173 --> ing19_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_7H2O -.-> CHEBI_32312
ing20_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010173 --> ing20_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_5H2O -.-> CHEBI_31440
ing21_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_010173 --> ing21_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_5H2O -.-> CHEBI_131361
ing22_CoSO4_7H2["CoSO4・7H2"]
CultureMech_010173 --> ing22_CoSO4_7H2
ing23_KAl_SO4_2_12H2O["KAl(SO4)2・12H2O"]
CultureMech_010173 --> ing23_KAl_SO4_2_12H2O
CHEBI_86465["CHEBI:86465"]
ing23_KAl_SO4_2_12H2O -.-> CHEBI_86465
ing24_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_010173 --> ing24_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing24_Nitrilotriacetic_acid -.-> CHEBI_44557
ing25_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010173 --> ing25_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing25_MnSO4_xH2O -.-> CHEBI_86360
ing26_KOH["KOH"]
CultureMech_010173 --> ing26_KOH
CHEBI_32035["CHEBI:32035"]
ing26_KOH -.-> CHEBI_32035
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.1, 3.0] |
| 3 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.45 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Fructose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 11 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
10 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
10 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
25 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
10 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 | CoSO4・7H2 | 0.18 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 24 |
KAl(SO4)2・12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
20 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 26 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
Trace element solution (see below)
Dose: 20 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 20 G_PER_L
Cross-reference to Medium M190
Na2CO3 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.669920Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M766 |
| 2026-01-28T19:48:34.453064Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 solution; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:57.221616+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:28.800128Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:11.851695Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010173 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.786000+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acetobacterium Medium" to "acetobacterium_medium" for consistent matching |
| 2026-05-16T09:03:20.678900+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.232769+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.868335+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.566192+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:52.236587+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |