Overview
- External ontology
- TOGO:M769 — Modified HB-1 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M769 Original source: JCM - JCM_M744 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=744
Composition
flowchart LR
CultureMech_010176["modified_hb_1_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010176 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_010176 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_010176 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_010176 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010176 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_010176 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_010176 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_H3BO3["H3BO3"]
CultureMech_010176 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_KCl["KCl"]
CultureMech_010176 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_010176 --> ing9_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing9_NiCl2_6H2O -.-> CHEBI_34887
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_010176 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_010176 --> ing11_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing11_Na2WO4_2H2O -.-> CHEBI_63939
ing12_NaBr["NaBr"]
CultureMech_010176 --> ing12_NaBr
CHEBI_63004["CHEBI:63004"]
ing12_NaBr -.-> CHEBI_63004
ing13_SrCl2_6H2O["SrCl2·6H2O"]
CultureMech_010176 --> ing13_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing13_SrCl2_6H2O -.-> CHEBI_36385
ing14_KI["KI"]
CultureMech_010176 --> ing14_KI
CHEBI_8346["CHEBI:8346"]
ing14_KI -.-> CHEBI_8346
ing15_NaOH["NaOH"]
CultureMech_010176 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010176 --> ing16_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing16_Carbon_dioxide_gas -.-> CHEBI_16526
ing17_N2["N2"]
CultureMech_010176 --> ing17_N2
CHEBI_17997["CHEBI:17997"]
ing17_N2 -.-> CHEBI_17997
ing18_Hydrogen_gas["Hydrogen gas"]
CultureMech_010176 --> ing18_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing18_Hydrogen_gas -.-> CHEBI_18276
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.75 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
0.015 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.325 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
10 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
SrCl2·6H2O
Cl2Sr.6H2O 266.616 g/mol
|
7.5 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
KI
I.K 166.002 g/mol
|
0.5 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 17 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 18 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 19 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
Na2S・9H2O solution
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
0.05 M PIPES (pH 7.0) solution
Dose: 25 G_PER_L
Role: Buffer; Properties: Solution, Organic compound, Defined component
10% KNO3 solution
Dose: 10 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
3% Na2S·9H2O solution (neutralized)
Dose: 4 G_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.678368Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M769 |
| 2026-01-28T19:48:19.373773Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:32.078920+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:01.279654Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:11.885452Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010176 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.819823+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified HB-1 Medium" to "modified_hb_1_medium" for consistent matching |
| 2026-05-16T09:03:20.714361+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.272775+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.909104+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.582996+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |