Overview
- External ontology
- TOGO:M772 — Freshwater Medium With Toluene
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M772 Original source: JCM - JCM_M747 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=747
Composition
flowchart LR
CultureMech_010180["freshwater_medium_with_toluene"]
ing0_Sol__1["Sol. 1"]
CultureMech_010180 --> ing0_Sol__1
ing1_Distilled_water["Distilled water"]
CultureMech_010180 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_NaCl["NaCl"]
CultureMech_010180 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010180 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010180 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_010180 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010180 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_010180 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Na2SO4["Na2SO4"]
CultureMech_010180 --> ing8_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing8_Na2SO4 -.-> CHEBI_32149
ing9_Iron_III__citrate["Iron(III) citrate"]
CultureMech_010180 --> ing9_Iron_III__citrate
CHEBI_144421["CHEBI:144421"]
ing9_Iron_III__citrate -.-> CHEBI_144421
ing10_Sodium_acetate_3H2O["Sodium acetate・3H2O"]
CultureMech_010180 --> ing10_Sodium_acetate_3H2O
CHEBI_32954["CHEBI:32954"]
ing10_Sodium_acetate_3H2O -.-> CHEBI_32954
ing11_NaOH["NaOH"]
CultureMech_010180 --> ing11_NaOH
CHEBI_32145["CHEBI:32145"]
ing11_NaOH -.-> CHEBI_32145
ing12_N2["N2"]
CultureMech_010180 --> ing12_N2
CHEBI_17997["CHEBI:17997"]
ing12_N2 -.-> CHEBI_17997
ing13_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010180 --> ing13_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing13_Carbon_dioxide_gas -.-> CHEBI_16526
ing14_Nitrogen_gas["Nitrogen gas"]
CultureMech_010180 --> ing14_Nitrogen_gas
ing14_Nitrogen_gas -.-> CHEBI_17997
ing15_Toluene["Toluene"]
CultureMech_010180 --> ing15_Toluene
CHEBI_17578["CHEBI:17578"]
ing15_Toluene -.-> CHEBI_17578
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 0.505 G_PER_L | ||
| 2 |
Distilled water
H2O 18.015 g/mol
|
970 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.42 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 |
Iron(III) citrate
C6H5FeO7 244.944 g/mol
|
12 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Simple component, Organic compound, Defined component |
| 11 |
Sodium acetate・3H2O
C2H3O2.Na 82.034 g/mol
|
1.36 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 12 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 13 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 15 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 16 |
Toluene
C7H8 92.141 g/mol
|
5 G_PER_L |
CHEBI:17578
toluene
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
Solutions
Solution A
Dose: 50 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
8.5% NaHCO3 solution
Dose: 30 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Vitamin solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Thiamine solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Vitamin B12 solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
100 mM FeCl2 solution
Dose: 0.5 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.684628Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M772 |
| 2026-01-28T19:48:27.107005Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:44.941244+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 9 solution(s) from ingredients |
| 2026-03-13T06:20:11.912022Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010180 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.846768+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Freshwater Medium With Toluene" to "freshwater_medium_with_toluene" for consistent matching |
| 2026-05-16T09:03:20.740378+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.303916+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.592559+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |