Overview
- External ontology
- TOGO:M773 — Metallosphaera Cuprina Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M773 Original source: JCM - JCM_M748 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=748
Composition
flowchart LR
CultureMech_010181["metallosphaera_cuprina_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010181 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010181 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_010181 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_010181 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_KCl["KCl"]
CultureMech_010181 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_010181 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Ca_NO3_2["Ca(NO3)2"]
CultureMech_010181 --> ing6_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing6_Ca_NO3_2 -.-> CHEBI_64205
ing7_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010181 --> ing7_Tryptone__BD_Difco_
ing8_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010181 --> ing8_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_2H2O -.-> CHEBI_75213
ing9_H3BO3["H3BO3"]
CultureMech_010181 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010181 --> ing10_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing10_CoCl2_6H2O -.-> CHEBI_35696
ing11_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010181 --> ing11_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_7H2O -.-> CHEBI_32312
ing12_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010181 --> ing12_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_5H2O -.-> CHEBI_31440
ing13_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_010181 --> ing13_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing13_FeCl3_6H2O -.-> CHEBI_86254
ing14_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010181 --> ing14_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing14_MnSO4_xH2O -.-> CHEBI_86360
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
0.01 G_PER_L |
CHEBI:64205
calcium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Tryptone (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 9 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.08 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.2 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.06 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.09 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.1 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution (filter--sterilized)
Dose: 2 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.687257Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M773 |
| 2026-01-28T19:48:13.262303Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:21.969778+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:50.093951Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.922015Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010181 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.857279+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Metallosphaera Cuprina Medium" to "metallosphaera_cuprina_medium" for consistent matching |
| 2026-05-16T09:03:20.752167+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:44.315728+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.945740+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:32.940561+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.598387+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:28.055794+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |