dethiosulfovibrio_ii_medium

Overview

External ontology

TOGO:M783 — Dethiosulfovibrio II Medium

Applications

• Microbial cultivation

pH range

{'min': 6.7, 'max': 6.8}

Notes

JCM Medium 758, MediaDive J758, and TOGO M783 describe Dethiosulfovibrio II Medium as a 1.034 L anoxic N2 medium containing 1.0 L distilled water, 1.0 ml each of FeCl2 solution and Trace element solution from JCM Medium 187, 0.5 mg resazurin, 2.0 ml 10% CaCl2 x 2 H2O, 20.0 ml 12.5% Na2S2O3 x 5 H2O, and 10.0 ml 5% Na2S x 9 H2O stocks.

Composition

flowchart LR
    CultureMech_010192["dethiosulfovibrio_ii_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_010192 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_010192 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_010192 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Trisodium_citrate_x_H2O["Trisodium citrate x H2O"]
    CultureMech_010192 --> ing3_Trisodium_citrate_x_H2O
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate_x_H2O -.-> CHEBI_53258
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_010192 --> ing4_Yeast_extract
    ing5_Bacto_peptone["Bacto peptone"]
    CultureMech_010192 --> ing5_Bacto_peptone
    ing6_Resazurin["Resazurin"]
    CultureMech_010192 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Distilled_water["Distilled water"]
    CultureMech_010192 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377

Ingredients (8)

Preparation

1. Mix main-solution components including FeCl2 solution, Trace element solution, and 967.118 ml/L distilled water under a N2 atmosphere.
2. Autoclave the main solution under a N2 atmosphere.
3. After cooling, add 1.93424 ml/L 10% CaCl2 x 2 H2O solution, 19.3424 ml/L 12.5% Na2S2O3 x 5 H2O solution, and 9.67118 ml/L 5% Na2S x 9 H2O solution from sterile anaerobic stocks stored under N2; the thiosulfate stock is filter-sterilized.
4. Adjust pH to 6.7-6.8 if necessary.

Solutions

Target organisms

No target organisms are recorded.

Provenance