Overview
- External ontology
- TOGO:M786 — Desulfurispirillum Alkaliphilum Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M786 Original source: JCM - JCM_M760-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=760
Composition
flowchart LR
CultureMech_010195["desulfurispirillum_alkaliphilum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010195 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010195 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_K2HPO4["K2HPO4"]
CultureMech_010195 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_NaHCO3["NaHCO3"]
CultureMech_010195 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Na2CO3["Na2CO3"]
CultureMech_010195 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_N2["N2"]
CultureMech_010195 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
ing6_NH4Cl["NH4Cl"]
CultureMech_010195 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_sulfur["sulfur"]
CultureMech_010195 --> ing7_sulfur
CHEBI_26833["CHEBI:26833"]
ing7_sulfur -.-> CHEBI_26833
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
6 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
8 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
22 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
sulfur
S 32.067 g/mol
|
2 G_PER_L |
CHEBI:26833
sulfur atom
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
1% Yeast extract solution
Dose: 2 G_PER_L
Role: Nutrient source; Properties: Undefined component
1.0 M Sodium acetate solution
Dose: 20 G_PER_L
Role: Buffer; Properties: Organic compound, Defined component, Solution
0.1 M MgSO4 solution
Dose: 10 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Defined component
SL--4 trace element solution (see Medium [M335])
Dose: 1 G_PER_L
Cross-reference to Medium M335
5% Na2S・9H2O solution
Dose: 2.5 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.712924Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M786 |
| 2026-01-28T19:48:07.035262Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:11.344439+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:20:12.035478Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010195 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.973243+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfurispirillum Alkaliphilum Medium" to "desulfurispirillum_alkaliphilum_medium" for consistent matching |
| 2026-05-16T09:03:20.882117+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.453293+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.645561+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |