mycoplasma_medium

Overview

External ontology

TOGO:M787 — Mycoplasma Medium

Applications

• Microbial cultivation

pH range

{'min': 7.2, 'max': 7.6}

Notes

MediaDive J761 and TOGO M787 describe JCM Medium 761 Mycoplasma Medium as a 1.0 L solid agar medium containing 700.0 ml distilled water, 17.5 g Heart Infusion Broth, 10.0 g Bacto agar, 200.0 ml heat-inactivated horse serum, and 100.0 ml 25% Yeast Extract Solution.

Composition

flowchart LR
    CultureMech_010196["mycoplasma_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010196 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Heart_Infusion_Broth["Heart Infusion Broth"]
    CultureMech_010196 --> ing1_Heart_Infusion_Broth
    ing2_Agar["Agar"]
    CultureMech_010196 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Horse_serum["Horse serum"]
    CultureMech_010196 --> ing3_Horse_serum

Ingredients (4)

Preparation

1. Dissolve Heart Infusion Broth and Agar in 700.0 ml/L distilled water.
2. Autoclave the basal agar at 121 C for 15 min.
3. Cool to 55 C and aseptically add 200.0 ml/L heat-inactivated horse serum and 100.0 ml/L filter-sterilized 25% Yeast Extract Solution warmed to 55 C.
4. Adjust final pH to 7.2-7.6.

Solutions

Target organisms

No target organisms are recorded.

Provenance