alkaline_tryptone_soya_broth_medium

CultureMech:010200 bacterial COMPLEX SOLID_AGAR
Original name: Alkaline Tryptone Soya Broth Medium

Overview

External ontology
TOGO:M790 — Alkaline Tryptone Soya Broth Medium
Applications
  • Microbial cultivation
pH range
{'min': 8.5, 'max': 9.0}
Notes
MediaDive J763 and TOGO M789 describe JCM Medium 763 Alkaline Tryptone Soya Broth Medium as 30.0 g/L Oxoid Tryptone soya broth in 1000.0 ml/L distilled water, adjusted after autoclaving to pH 8.5-9.0 with sterile autoclaved 20% sodium carbonate solution. TOGO M790 additionally applies the 20.0 g/L agar solidification step.

Composition

flowchart LR
    CultureMech_010200["alkaline_tryptone_soya_broth_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010200 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Tryptone_soya_broth__Oxoid_["Tryptone soya broth (Oxoid)"]
    CultureMech_010200 --> ing1_Tryptone_soya_broth__Oxoid_
    ing2_Agar["Agar"]
    CultureMech_010200 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 TOGO M790 / JCM Medium 763 contributes 1000.0 ml/L distilled water.
2 Tryptone soya broth (Oxoid) 30.0 G_PER_L TOGO M790 / JCM Medium 763 lists 30.0 g/L Oxoid Tryptone soya broth.
3 Agar 20.0 G_PER_L CHEBI:2509
agar
 TOGO M790 / JCM Medium 763 adds 20.0 g/L agar for solid medium.

Preparation

  1. Dissolve 30.0 g/L Oxoid Tryptone soya broth and 20.0 g/L agar in 1000.0 ml/L water.
  2. Autoclave.
  3. After cooling, adjust pH to 8.5-9.0 with sterile autoclaved 20% sodium carbonate solution.

Solutions

20% sodium carbonate solution

Dose: variable VARIABLE

Stock composition
IngredientConcentrationNotes
Na2CO3 20.0 PERCENT_W_V TOGO M790 / JCM Medium 763 specifies the pH-adjusting sodium carbonate solution as 20.0% w/v.
TOGO M790 / JCM Medium 763 uses sterile 20% sodium carbonate to adjust pH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.718706Z togo-import Imported from TOGO Medium Source: JCM, ID: M790
2026-01-28T19:48:07.249395Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: sodium carbonate solution
2026-03-09T08:04:11.693668+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:12.055964Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010200
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.995306+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Alkaline Tryptone Soya Broth Medium" to "alkaline_tryptone_soya_broth_medium" for consistent matching
2026-05-16T09:03:20.902132+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:44.474518+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m789_alkaline_tsb_score15.py RESOLVED_TOGO_M790_ALKALINE_TRYPTONE_SOYA_BROTH_SOLID Moved sodium carbonate solution from an empty solution stub into a structured 20% stock used as a variable pH adjuster, corrected water units and agar naming, added the final pH range, and linked the MediaDive J763 liquid parent.