Overview
- External ontology
- TOGO:M797 — Modified R2A Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M797 Original source: JCM - JCM_M769-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=769
Composition
flowchart LR
CultureMech_010207["modified_r2a_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010207 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010207 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_010207 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_010207 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_010207 --> ing4_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Sodium_pyruvate -.-> CHEBI_50144
ing5_Trisodium_citrate["Trisodium citrate"]
CultureMech_010207 --> ing5_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Trisodium_citrate -.-> CHEBI_53258
ing6_Glucose["Glucose"]
CultureMech_010207 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_washed_agar["washed agar"]
CultureMech_010207 --> ing7_washed_agar
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010207 --> ing8_Yeast_extract__BD_Difco_
ing9_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_010207 --> ing9_Proteose_peptone_No__3__BD_Difco_
ing10_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010207 --> ing10_Casamino_acids__BD_Difco_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
230 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 6 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 8 | washed agar | 18 G_PER_L | Role: Solidifying component | |
| 9 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 10 | Proteose peptone No. 3 (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 11 | Casamino acids (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.728480Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M797 |
| 2026-01-28T19:48:39.028684Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:12.101110Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010207 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.042575+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified R2A Medium" to "modified_r2a_medium" for consistent matching |
| 2026-06-05T07:40:44.515099+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.067729+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.663835+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |