Overview
- External ontology
- TOGO:M79 — 1% Ogawa Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M79 Original source: JCM - JCM_M88 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=88
Composition
flowchart LR
CultureMech_010210["1_ogawa_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010210 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_010210 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Sodium_glutamate["Sodium glutamate"]
CultureMech_010210 --> ing2_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing2_Sodium_glutamate -.-> CHEBI_64220
ing3_Glycerol["Glycerol"]
CultureMech_010210 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_Whole_egg["Whole egg"]
CultureMech_010210 --> ing4_Whole_egg
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
10 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component |
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
6 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 5 | Whole egg | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component |
Solutions
Solution I
Dose: 1 G_PER_L
See mediadive_5335_Solution_I.yaml for composition
Solution II
Dose: 212 G_PER_L
See mediadive_5336_Solution_II.yaml for composition
2% Malachite green solution
Dose: 6 G_PER_L
Role: Selective agent, pH indicator; Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.717444Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M79 |
| 2026-01-28T19:48:13.186315Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:21.873268+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:12.133987Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010210 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.075337+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1% Ogawa Medium" to "1_ogawa_medium" for consistent matching |
| 2026-05-16T09:03:20.972238+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:44.553474+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.002124+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:51.676725+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |