Overview
- External ontology
- TOGO:M819 — Natronoarchaeum Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M819 Original source: JCM - JCM_M788 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=788
Composition
flowchart LR
CultureMech_010231["natronoarchaeum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010231 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010231 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_K2HSO4["K2HSO4"]
CultureMech_010231 --> ing2_K2HSO4
ing3_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010231 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_010231 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010231 --> ing5_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_4H2O -.-> CHEBI_86368
ing6_Sodium_glutamate["Sodium glutamate"]
CultureMech_010231 --> ing6_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing6_Sodium_glutamate -.-> CHEBI_64220
ing7_Trisodium_citrate["Trisodium citrate"]
CultureMech_010231 --> ing7_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing7_Trisodium_citrate -.-> CHEBI_53258
ing8_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_010231 --> ing8_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_4H2O -.-> CHEBI_86249
ing9_Glucose["Glucose"]
CultureMech_010231 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010231 --> ing10_Yeast_extract__BD_Difco_
ing11_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010231 --> ing11_Casamino_acids__BD_Difco_
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
220 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | K2HSO4 | 1 G_PER_L | Role: Buffer; Properties: Defined component, Inorganic compound, Simple component | |
| 4 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
0.1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Simple component, Defined component, Organic compound |
| 8 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 9 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 11 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 12 | Casamino acids (BD-Difco) | 4 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.776391Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M819 |
| 2026-01-28T19:48:21.183940Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:12.307497Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010231 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.249727+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronoarchaeum Medium" to "natronoarchaeum_medium" for consistent matching |
| 2026-06-05T07:40:44.756572+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |