mh1_medium_for_halarchaeum

CultureMech:010234 archaea COMPLEX LIQUID
Original name: MH1 Medium For Halarchaeum

Overview

External ontology
TOGO:M821 — MH1 Medium For Halarchaeum
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M821 Original source: JCM - JCM_M789 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=789

Composition

flowchart LR
    CultureMech_010234["mh1_medium_for_halarchaeum"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010234 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_010234 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KH2PO4["KH2PO4"]
    CultureMech_010234 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_010234 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010234 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_K2SO4["K2SO4"]
    CultureMech_010234 --> ing5_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing5_K2SO4 -.-> CHEBI_32036
    ing6_L__Glutamic_acid["L--Glutamic acid"]
    CultureMech_010234 --> ing6_L__Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing6_L__Glutamic_acid -.-> CHEBI_16015
    ing7_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
    CultureMech_010234 --> ing7_Trisodium_citrate_2H2O
    CHEBI_53258["CHEBI:53258"]
    ing7_Trisodium_citrate_2H2O -.-> CHEBI_53258
    ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010234 --> ing8_Yeast_extract__BD_Difco_
    ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_010234 --> ing9_Casamino_acids__BD_Difco_
    ing10_KOH["KOH"]
    CultureMech_010234 --> ing10_KOH
    CHEBI_32035["CHEBI:32035"]
    ing10_KOH -.-> CHEBI_32035

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
5 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 K2SO4
2K.O4S 174.259 g/mol
5 G_PER_L CHEBI:32036
potassium sulfate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 L--Glutamic acid
C5H9NO4 147.13 g/mol
2 G_PER_L CHEBI:16015
L-glutamic acid
 Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component
8 Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
2 G_PER_L CHEBI:53258
sodium citrate
 Role: Buffer; Properties: Simple component, Organic compound, Defined component
9 Yeast extract (BD-Difco) 2 G_PER_L Properties: Undefined component
10 Casamino acids (BD-Difco) 4 G_PER_L Properties: Undefined component
11 KOH
HO.K 56.105 g/mol
variable VARIABLE CHEBI:32035
potassium hydroxide
 Role: Mineral source; Properties: Defined component, Solution

Solutions

0.2% FeSO4・6H2O solution

Dose: 2 G_PER_L

Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

Trace metal solution (see Medium [M805])

Dose: 2 G_PER_L

Cross-reference to Medium M805

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.781347Z togo-import Imported from TOGO Medium Source: JCM, ID: M821
2026-01-28T19:48:41.495276Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: KOH
2026-03-09T08:05:09.993035+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:12.327400Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010234
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.270705+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MH1 Medium For Halarchaeum" to "mh1_medium_for_halarchaeum" for consistent matching
2026-05-16T09:03:21.182497+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:44.781004+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.745754+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.