m_tge_broth_medium

CultureMech:010242 bacterial COMPLEX SOLID_AGAR
Original name: m TGE Broth Medium

Overview

External ontology
TOGO:M829 — m TGE Broth Medium
Applications
  • Microbial cultivation
Notes
TOGO M829 mirrors the solid JCM Medium 795 preparation: 18.0 g Bacto m TGE broth (BD-Difco), 15.0 g/L agar, and 1.0 L distilled water.

Composition

flowchart LR
    CultureMech_010242["m_tge_broth_medium"]
    ing0_Bacto_m_TGE_broth__BD_Difco_["Bacto m TGE broth (BD-Difco)"]
    CultureMech_010242 --> ing0_Bacto_m_TGE_broth__BD_Difco_
    ing1_Distilled_water["Distilled water"]
    CultureMech_010242 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_agar["agar"]
    CultureMech_010242 --> ing2_agar
    CHEBI_2509["CHEBI:2509"]
    ing2_agar -.-> CHEBI_2509

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Bacto m TGE broth (BD-Difco) 18.0 G_PER_L JCM Medium 795 lists 18.0 g Bacto m TGE broth (BD-Difco).
2 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
JCM Medium 795 lists 1.0 L distilled water.
3 agar 15.0 G_PER_L CHEBI:2509
agar
JCM Medium 795 adds 15.0 g/L agar for solid medium.

Preparation

  1. Suspend 18.0 g Bacto m TGE broth (BD-Difco) and 15.0 g agar in 1.0 L distilled water.
  2. Autoclave at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.793526Z togo-import Imported from TOGO Medium Source: JCM, ID: M829
2026-01-28T19:48:10.187386Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:12.378287Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010242
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.321972+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "m TGE Broth Medium" to "m_tge_broth_medium" for consistent matching
2026-06-05T07:40:44.839964+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_jcm_wrappers_direct_score20.py RESOLVED_JCM_WRAPPERS_DIRECT_SCORE20 TOGO M829 mirrors the solid JCM Medium 795 preparation: 18.0 g Bacto m TGE broth (BD-Difco), 15.0 g/L agar, and 1.0 L distilled water.