Overview
- External ontology
- TOGO:M836 — BHI-S Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M836 Original source: JCM - JCM_M801 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=801 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_010250["bhi_s_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010250 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010250 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Resazurin["Resazurin"]
CultureMech_010250 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Na2S_9H2O["Na2S・9H2O"]
CultureMech_010250 --> ing3_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_9H2O -.-> CHEBI_76209
ing4_Sulfur__powder_["Sulfur (powder)"]
CultureMech_010250 --> ing4_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing4_Sulfur__powder_ -.-> CHEBI_33403
ing5_PIPES["PIPES"]
CultureMech_010250 --> ing5_PIPES
CHEBI_39033["CHEBI:39033"]
ing5_PIPES -.-> CHEBI_39033
ing6_Calf_brains["Calf brains"]
CultureMech_010250 --> ing6_Calf_brains
ing7_Beef_heart["Beef heart"]
CultureMech_010250 --> ing7_Beef_heart
ing8_Proteose_peptone["Proteose peptone"]
CultureMech_010250 --> ing8_Proteose_peptone
ing9_Dextrose["Dextrose"]
CultureMech_010250 --> ing9_Dextrose
CHEBI_17634["CHEBI:17634"]
ing9_Dextrose -.-> CHEBI_17634
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_010250 --> ing10_Sodium_chloride
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Disodium_phosphate["Disodium phosphate"]
CultureMech_010250 --> ing11_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing11_Disodium_phosphate -.-> CHEBI_34683
ing12_N2["N2"]
CultureMech_010250 --> ing12_N2
CHEBI_17997["CHEBI:17997"]
ing12_N2 -.-> CHEBI_17997
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
23 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 4 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component |
| 5 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 | PIPES | 6.05 G_PER_L |
CHEBI:39033
PIPES
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 7 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 8 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 9 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 10 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 12 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 13 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.803235Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M836 |
| 2026-01-28T19:48:34.779224Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:12.424403Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010250 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.367396+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BHI-S Medium" to "bhi_s_medium" for consistent matching |
| 2026-05-16T09:03:21.275082+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:44.894405+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |