Overview
- External ontology
- TOGO:M840 — 1/10 R2A Broth
- Applications
-
- Microbial cultivation
- Notes
- TOGO M840 mirrors JCM Medium 805: 0.32 g R2A broth DAIGO per 1.0 L distilled water, a 1:10 dilution of JCM Medium 839 R2A Broth DAIGO.
Composition
flowchart LR
CultureMech_010255["1_10_r2a_broth"]
ing0_R2A_broth__DAIGO___Nihon_Pharm__Co__["R2A broth (DAIGO) (Nihon Pharm. Co.)"]
CultureMech_010255 --> ing0_R2A_broth__DAIGO___Nihon_Pharm__Co__
ing1_Distilled_water["Distilled water"]
CultureMech_010255 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | R2A broth (DAIGO) (Nihon Pharm. Co.) | 0.32 G_PER_L | JCM Medium 805 lists 0.32 g R2A broth DAIGO in 1.0 L distilled water. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 805 lists 1.0 L distilled water. |
Preparation
- Dissolve 0.32 g R2A broth (DAIGO) in 1.0 L distilled water.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.808435Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M840 |
| 2026-01-28T19:48:24.033896Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:12.442681Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010255 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.385783+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/10 R2A Broth" to "1_10_r2a_broth" for consistent matching |
| 2026-06-05T07:40:44.917819+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-06T00:00:00-07:00 | repair_r2a_score40.py | RESOLVED_R2A_SCORE40_GRAPH | TOGO M840 mirrors JCM Medium 805: 0.32 g R2A broth DAIGO per 1.0 L distilled water, a 1:10 dilution of JCM Medium 839 R2A Broth DAIGO. |
| 2026-10-07T07:16:23Z | codex | CURATED_CONCENTRATION_EVIDENCE | Exact source snippets and claim-specific calculations attached in YAML. Batch volumes remain separate from stock strengths. Unresolved final-volume claims were not altered. |