Overview
- External ontology
- TOGO:M850 — Acidic Diluted ASM Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M850 Original source: JCM - JCM_M815 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=815
Composition
flowchart LR
CultureMech_010266["acidic_diluted_asm_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010266 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010266 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010266 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_010266 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_HCl["HCl"]
CultureMech_010266 --> ing4_HCl
CHEBI_17883["CHEBI:17883"]
ing4_HCl -.-> CHEBI_17883
ing5_Methane_gas["Methane gas"]
CultureMech_010266 --> ing5_Methane_gas
ing6_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010266 --> ing6_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_2H2O -.-> CHEBI_75213
ing7_H3BO3["H3BO3"]
CultureMech_010266 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010266 --> ing8_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_4H2O -.-> CHEBI_86368
ing9_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010266 --> ing9_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing9_CoCl2_6H2O -.-> CHEBI_35696
ing10_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010266 --> ing10_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_6H2O -.-> CHEBI_34887
ing11_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010266 --> ing11_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_7H2O -.-> CHEBI_32312
ing12_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010266 --> ing12_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_5H2O -.-> CHEBI_31440
ing13_EDTA_2Na["EDTA・2Na"]
CultureMech_010266 --> ing13_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing13_EDTA_2Na -.-> CHEBI_64734
ing14_EDTA_Fe_III_["EDTA・Fe(III)"]
CultureMech_010266 --> ing14_EDTA_Fe_III_
ing15_KH2PO4["KH2PO4"]
CultureMech_010266 --> ing15_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing15_KH2PO4 -.-> CHEBI_63036
ing16_Na2HPO4_2H2O["Na2HPO4・2H2O"]
CultureMech_010266 --> ing16_Na2HPO4_2H2O
CHEBI_34683["CHEBI:34683"]
ing16_Na2HPO4_2H2O -.-> CHEBI_34683
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 4 identical duplicates: 1.0, 1.0, 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
| 6 | Methane gas | variable VARIABLE | Properties: Gas | |
| 7 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.04 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
0.015 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.02 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.05 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.4 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 15 | EDTA・Fe(III) | 4.5 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 16 |
KH2PO4
H2O4P.K 136.084 g/mol
|
37.425 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2HPO4・2H2O
HO4P.2Na 141.958 g/mol
|
48.95 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution (see below)
Dose: 0.1 G_PER_L
Iron stock solution (see below)
Dose: 0.1 G_PER_L
Phosphate buffer stock solution
Dose: 2 G_PER_L
See mediadive_4768_Phosphate_buffer_stock_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.828925Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M850 |
| 2026-01-28T19:48:36.784737Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl; Added default concentration for ingredient: Methane gas |
| 2026-03-09T08:05:01.392479+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:33.243012Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:12.534359Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010266 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.477831+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acidic Diluted ASM Medium" to "acidic_diluted_asm_medium" for consistent matching |
| 2026-05-16T09:03:21.384166+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.014556+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.445126+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.812685+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:52.920794+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |
| 2026-09-06T00:00:00-07:00 | repair_jcm_reference_wrappers_score40.py | LINKED_JCM_REFERENCE_WRAPPER_CHILD | Added or refreshed reciprocal parent-child link for bacterial/TOGO_M851_Diluted_ASM_Medium.yaml. |