Overview
- External ontology
- TOGO:M852 — Low-Salt Methanotrophic Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M852 Original source: JCM - JCM_M817 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=817
Composition
flowchart LR
CultureMech_010268["low_salt_methanotrophic_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010268 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010268 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010268 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KNO3["KNO3"]
CultureMech_010268 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_KBr["KBr"]
CultureMech_010268 --> ing4_KBr
CHEBI_32030["CHEBI:32030"]
ing4_KBr -.-> CHEBI_32030
ing5_Biotin["Biotin"]
CultureMech_010268 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_010268 --> ing6_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing6_p__Aminobenzoic_acid -.-> CHEBI_30753
ing7_Thiamine_HCl["Thiamine・HCl"]
CultureMech_010268 --> ing7_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing7_Thiamine_HCl -.-> CHEBI_49105
ing8_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_010268 --> ing8_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing8_Pyridoxine_HCl -.-> CHEBI_30961
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_010268 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
ing10_Riboflavin["Riboflavin"]
CultureMech_010268 --> ing10_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing10_Riboflavin -.-> CHEBI_17015
ing11_Nicotinic_acid["Nicotinic acid"]
CultureMech_010268 --> ing11_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing11_Nicotinic_acid -.-> CHEBI_15940
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KBr
Br.K 119.002 g/mol
|
0.1 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
| 7 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
10 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Simple component, Organic compound, Defined component |
| 8 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
10 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 9 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 10 | Vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Simple component, Organic compound, Defined component |
| 11 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
20 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
| 12 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
20 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
Solutions
Trace element solution (see Medium [M850])
Dose: 0.1 G_PER_L
Cross-reference to Medium M850
Iron stock solution (see Medium [M850])
Dose: 0.1 G_PER_L
Cross-reference to Medium M850
Vitamin solution* (see below)
Dose: 0.1 G_PER_L
Phosphate buffer stock solution (see Medium [M850])
Dose: 1 G_PER_L
Cross-reference to Medium M850
Selenite--tungstate solution (see Medium [M431])
Dose: 0.1 G_PER_L
Cross-reference to Medium M431
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.832135Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M852 |
| 2026-01-28T19:48:18.388071Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:30.490566+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:08:59.475746Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:12.546035Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010268 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.489844+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Low-Salt Methanotrophic Medium" to "low_salt_methanotrophic_medium" for consistent matching |
| 2026-05-16T09:03:21.398746+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.026438+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.042033+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:33.459300+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.817569+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:52.959104+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |