fermentative_cellulolytic_anaerobes_medium

CultureMech:010273 bacterial COMPLEX LIQUID
Original name: Fermentative Cellulolytic Anaerobes Medium

Overview

External ontology
TOGO:M857 — Fermentative Cellulolytic Anaerobes Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M857 Original source: JCM - JCM_M822 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=822

Composition

flowchart LR
    CultureMech_010273["fermentative_cellulolytic_anaerobes_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010273 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010273 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_010273 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_010273 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010273 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_010273 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_010273 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_KCl["KCl"]
    CultureMech_010273 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_MOPS["MOPS"]
    CultureMech_010273 --> ing8_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing8_MOPS -.-> CHEBI_39074
    ing9_1_0_M_Phosphate_buffer__see_below_["1.0 M Phosphate buffer (see below)"]
    CultureMech_010273 --> ing9_1_0_M_Phosphate_buffer__see_below_
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010273 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010273 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_5__L__Cysteine_HCl_H2O["5% L--Cysteine・HCl・H2O"]
    CultureMech_010273 --> ing12_5__L__Cysteine_HCl_H2O
    ing13_KH2PO4["KH2PO4"]
    CultureMech_010273 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_Na2HPO4["Na2HPO4"]
    CultureMech_010273 --> ing14_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing14_Na2HPO4 -.-> CHEBI_34683

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
910 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 Yeast extract 0.2 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
0.4 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
7 Resazurin
C12H7NO4 229.191 g/mol
0.25 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
8 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9 MOPS 2.09 G_PER_L CHEBI:39074
MOPS
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
10 1.0 M Phosphate buffer (see below) 1 G_PER_L
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
13 5% L--Cysteine・HCl・H2O 10 G_PER_L Properties: Defined component, Organic compound, Solution
14 KH2PO4
H2O4P.K 136.084 g/mol
50 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Solution
15 Na2HPO4
HO4P.2Na 141.958 g/mol
50 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Solution

Solutions

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

5% NaHCO3 solution*

Dose: 10 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

10% Cellobiose solution*

Dose: 40 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

5% Na2S・9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.842271Z togo-import Imported from TOGO Medium Source: JCM, ID: M857
2026-01-28T19:48:40.880344Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:05:08.819500+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T06:20:12.589759Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010273
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.535430+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Fermentative Cellulolytic Anaerobes Medium" to "fermentative_cellulolytic_anaerobes_medium" for consistent matching
2026-05-16T09:03:21.445471+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.076112+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:33.503302+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.837645+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.