Overview
- External ontology
- TOGO:M863 — Modified NMS Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M863 Original source: JCM - JCM_M828 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=828
Composition
flowchart LR
CultureMech_010280["modified_nms_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010280 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010280 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010280 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KNO3["KNO3"]
CultureMech_010280 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_Methane_gas["Methane gas"]
CultureMech_010280 --> ing4_Methane_gas
ing5_Air["Air"]
CultureMech_010280 --> ing5_Air
ing6_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_010280 --> ing6_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_6H2O -.-> CHEBI_86254
ing7_EDTA_2Na["EDTA・2Na"]
CultureMech_010280 --> ing7_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing7_EDTA_2Na -.-> CHEBI_64734
ing8_KH2PO4["KH2PO4"]
CultureMech_010280 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_Na2HPO4_2H2O["Na2HPO4・2H2O"]
CultureMech_010280 --> ing9_Na2HPO4_2H2O
CHEBI_34683["CHEBI:34683"]
ing9_Na2HPO4_2H2O -.-> CHEBI_34683
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1100.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 900.0, 100.0, 100.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 | Methane gas | variable VARIABLE | Properties: ガス | |
| 6 | Air | variable VARIABLE | Properties: ガス | |
| 7 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.027 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
0.0372 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.272 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 10 |
Na2HPO4・2H2O
HO4P.2Na 141.958 g/mol
|
0.717 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
Solutions
SL--4 trace element solution (see Medium [M335])
Dose: 5 G_PER_L
Cross-reference to Medium M335
Chelated iron solution (see below)
Dose: 10 G_PER_L
Phosphate buffer solution (see below)
Dose: 100 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.858278Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M863 |
| 2026-01-28T19:48:18.443840Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Methane gas; Added default concentration for ingredient: Air |
| 2026-03-09T08:04:30.586763+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:59.573735Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:12.661171Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010280 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.608315+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified NMS Medium" to "modified_nms_medium" for consistent matching |
| 2026-05-16T09:03:21.525354+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.159199+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.566023+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.871849+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |